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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2017

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes
Remarks:
ISO medium

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
other: ISO medium
Total exposure duration:
48 h

Test conditions

pH:
The pH of aerated water was in the range of 6 - 9.
Dissolved oxygen:
Rhe dissolved oxygen was ≥ 3 mg/L.
Nominal and measured concentrations:
A static dose response test was conducted at nominal concentrations of 4.27 - 9.39 - 20.66 - 45.45 - 100.00 μg/L of test item in ISO medium, with an untreated group (without test item).
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC10
Effect conc.:
8.31 µg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
19.7 µg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
4.27 µg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
LOEC
Effect conc.:
9.39 µg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The acute immobilisation test was performed to assess the effects of test item Basic Yellow 1 on Daphnia magna after 48 hours of exposure under static conditions.
20 daphnids, less than 24 hours old, were exposed to 4.27 - 9.39 - 20.66 - 45.45 - 100.00 μg/L of test item in medium.
Moreover an untreated group (without test item) was be run in addition to the treatment groups.
The assessment of the effects was based on the nominal concentrations of test item and active ingredient Thioflavine.
After 48 hours, the endpoints based on the test item nominal concentrations were estimated to be the following:

EC10 8.31 μg/L; EC20 11.17 μg/L; EC50 19.70 μg/L;
LOEC: 9.39 μg/L;
NOEC: 4.27 μg/L.

After 48 hours, the endpoints based on the nominal concentrations of active ingredient Thioflavine were estimated to be the following:

EC10 6.73 μg a.i./L; EC20 9.04 μg a.i./L; EC50 15.95 μg a.i./L;
LOEC: 7.60 μg a.i./L;
NOEC: 3.46 μg a.i./L.

No other effects were observed on the exposed organisms.
Executive summary:

The acute immobilisation test was performed to assess the effects of test item Basic Yellow 1 on Daphnia magna after 48 hours of exposure under static conditions.

20 daphnids, less than 24 hours old, were exposed to 4.27 - 9.39 - 20.66 - 45.45 - 100.00 μg/L of test item in medium.

Moreover an untreated group (without test item) was be run in addition to the treatment groups.

The assessment of the effects was based on the nominal concentrations of test item and active ingredient Thioflavine.

After 48 hours, the endpoints based on the test item nominal concentrations were estimated to be the following:

EC10 8.31 μg/L; EC20 11.17 μg/L; EC50 19.70 μg/L;

LOEC: 9.39 μg/L;

NOEC: 4.27 μg/L.

After 48 hours, the endpoints based on the nominal concentrations of active ingredient Thioflavine were estimated to be the following:

EC10 6.73 μg a.i./L; EC20 9.04 μg a.i./L; EC50 15.95 μg a.i./L;

LOEC: 7.60 μg a.i./L;

NOEC: 3.46 μg a.i./L.

No other effects were observed on the exposed organisms.