Registration Dossier
Registration Dossier
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EC number: 219-228-9 | CAS number: 2390-54-7
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2-[4-(dimethylamino)phenyl]-3,6-dimethylbenzothiazolium chloride
- EC Number:
- 219-228-9
- EC Name:
- 2-[4-(dimethylamino)phenyl]-3,6-dimethylbenzothiazolium chloride
- Cas Number:
- 2390-54-7
- Molecular formula:
- C17H19N2S.Cl
- IUPAC Name:
- 2-[4-(dimethylamino)phenyl]-3,6-dimethylbenzothiazolium chloride
- Test material form:
- solid: particulate/powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- yes
- Remarks:
- EPA medium
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
Study design
- Test type:
- not specified
- Water media type:
- other: EPA medium
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 22.4 - 23.0 °C
- pH:
- The pH values, measured at the beginning and at the end of the study, were in the range of 7.40 - 7.59 in the untreated group and 7.32 - 7.71 in the treated groups.
- Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Remarks:
- yield value
- Effect conc.:
- 9.27 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield value
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Remarks:
- growth rate
- Effect conc.:
- 29.79 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 4.58 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.43 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The growth inhibition test was performed in order to evaluate the effects of the test item Basic Yellow 1 on algae, after 72 hours of exposure.
Algal cultures were exposed to 1.43 - 4.58 - 14.65 - 46.88 - 150.00 μg test item/L, plus an untreated group.
The assessment of the effects was based on nominal concentrations of test item and geometric mean measured concentrations of active ingredient Thioflavine.
After 72 hours, the endpoints based on the nominal concentrations of test item Basic Yellow 1 were estimated to be the following:
EyC10 2.26 μg/L, EyC20 3.67 μg/L, EyC50 9.27 μg/L;
LOEyC 4.58 μg/L and NOEyC 1.43 μg/L;
ErC10 7.98 μg/L, ErC20 12.54 μg/L, ErC50 29.79 μg/L;
LOErC 4.58 μg/L and NOErC 1.43 μg/L.
After 72 hours, the endpoints based on the geometric mean measured concentrations of active ingredient Thioflavine were estimated to be the following:
EyC10 1.23 μg a.i./L, EyC20 2.09 a.i./L, EyC50 5.72 μg a.i./L;
LOEyC 2.44 a.i./L and NOEyC 0.94 a.i./L;
ErC10 5.81 a.i./L, ErC20 8.74 a.i./L, ErC50 19.09 μg a.i./L;
LOErC 2.44 a.i./L and NOErC 0.94 a.i./L.
At the test item concentrations 46.88 and 150.00 μg/L, cells with abnormal appearance was observed. - Executive summary:
The growth inhibition test was performed in order to evaluate the effects of the test item Basic Yellow 1 on algae, after 72 hours of exposure.
Algal cultures were exposed to 1.43 - 4.58 - 14.65 - 46.88 - 150.00 μg test item/L, plus an untreated group.
The assessment of the effects was based on nominal concentrations of test item and geometric mean measured concentrations of active ingredient Thioflavine.
After 72 hours, the endpoints based on the nominal concentrations of test item Basic Yellow 1 were estimated to be the following:
EyC10 2.26 μg/L, EyC20 3.67 μg/L, EyC50 9.27 μg/L;
LOEyC 4.58 μg/L and NOEyC 1.43 μg/L;
ErC10 7.98 μg/L, ErC20 12.54 μg/L, ErC50 29.79 μg/L;
LOErC 4.58 μg/L and NOErC 1.43 μg/L.
After 72 hours, the endpoints based on the geometric mean measured concentrations of active ingredient Thioflavine were estimated to be the following:
EyC10 1.23 μg a.i./L, EyC20 2.09 a.i./L, EyC50 5.72 μg a.i./L;
LOEyC 2.44 a.i./L and NOEyC 0.94 a.i./L;
ErC10 5.81 a.i./L, ErC20 8.74 a.i./L, ErC50 19.09 μg a.i./L;
LOErC 2.44 a.i./L and NOErC 0.94 a.i./L.
At the test item concentrations 46.88 and 150.00 μg/L, cells with abnormal appearance was observed.
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