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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2017

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
15 mg/L
Based on:
TOC
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
CO2 evolution
Reference substance
Reference substance:
acetic acid, sodium salt

Results and discussion

% Degradation
Parameter:
% degradation (CO2 evolution)
Value:
ca. -8.33
Sampling time:
28 d

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable
Conclusions:
The ready biodegradability of BASIC YELLOW 1 exposed to microorganisms derived from the activated sludge of a municipal sewage treatment was investigated under aerobic static exposure conditions, following the TG OECD 301b according to GLP regulations.
The biodegradation, based on CO2 evolution, of BASIC YELLOW 1 was calculated to be -8.33% after an incubation time of 28 days. Based on the results of this test, it is possible to conclude that BASIC YELLOW 1 isn’t readly biodegradable.
The Reference item Sodium acetate, reached the pass level of 60% (actual value 67.36%) of biodegradation after 14 days, thus confirming suitability of inoculum and test condition.
In the Toxicity control containing both the Test item and reference item, the % of Biodegradation occurred within 14 days was 32.68%, therefore the test item was found to have no inhibiting effect on microbial activity at the concentration used in present study.
The Abiotic sterile control (obtained using a sterilised inoculated solution of HgCl2 [10g/l]), confirmed that the test item was not abiotically degraded.
Executive summary:

The ready biodegradability of BASIC YELLOW 1 exposed to microorganisms derived from the activated sludge of a municipal sewage treatment was investigated under aerobic static exposure conditions, following the TG OECD 301b according to GLP regulations.

The biodegradation, based on CO2 evolution, of BASIC YELLOW 1 was calculated to be -8.33% after an incubation time of 28 days. Based on the results of this test, it is possible to conclude that BASIC YELLOW 1 isn’t readly biodegradable.

The Reference item Sodium acetate, reached the pass level of 60% (actual value 67.36%) of biodegradation after 14 days, thus confirming suitability of inoculum and test condition.

In the Toxicity control containing both the Test item and reference item, the % of Biodegradation occurred within 14 days was 32.68%, therefore the test item was found to have no inhibiting effect on microbial activity at the concentration used in present study.

The Abiotic sterile control (obtained using a sterilised inoculated solution of HgCl2 [10g/l]), confirmed that the test item was not abiotically degraded.