Registration Dossier

Administrative data

Endpoint:
partition coefficient
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2017
Report Date:
2017

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 117 (Partition Coefficient (n-octanol / water), HPLC Method)
GLP compliance:
yes (incl. certificate)
Type of method:
HPLC method
Partition coefficient type:
octanol-water

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder
Specific details on test material used for the study:
Batch Number: 161217

Study design

Analytical method:
high-performance liquid chromatography

Results and discussion

Partition coefficient
Type:
log Pow
Partition coefficient:
3.02
Temp.:
25 °C
pH:
7.98

Any other information on results incl. tables

RESULTS EVALUATION:

The retention times of reference and test item were determined and the capacity factor (k’) were

calculated from the retention of the substance concerned (tr) and the unretained component (t0): k' = (tr - t0)/t0

The corresponding logarithm of the capacity factor of reference item was calculated and a correlation

graphs of log k’ versus log Pow was constructed.

The partition coefficient of test item was obtained by interpolation of the calculated capacity factor on

the calibration curve.

Therefore the log Pow value x of the test item was calculated as follows:

y = m * x + b

where:

y = log k' (logarithm of capacity factor)

x = log Pow(value of the test item)

m = slope derived from the linear regression of reference items

b = y-axis intercept derived from the linear regression of reference items

Log Pow = (log k' – b) / m

CAPACITY FACTOR K' AND LOG Pow OF TEST ITEM

Concentration

(mg/L)

Injection

Retention

time (min)

 

Capacity

Factor

Log k’ 

Log POW

 53.8

 1

 6.43

 1.81

 0.26

 3.01

 2

 6.48

 1.83

 0.26

 3.03

 

 

 

Average

values

 6.46

 1.82

 0.26

 3.02

Applicant's summary and conclusion

Conclusions:
Log Pow of the substance at 25°C is 3.02 (pH of eluent solution=7.98).
Executive summary:

Partition coefficient of the test item was determined by HPLC method. Log Pow of the substance at 25°C was 3.02 (pH of eluent solution=7.98).