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Biodegradation in water: screening tests

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Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1982
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
None
Qualifier:
according to
Guideline:
OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
Version / remarks:
OECD Test Method 301 A, May 12, 1981
Deviations:
yes
Remarks:
Bacteria counted by plating: 4.3E+6/ml
GLP compliance:
no
Specific details on test material used for the study:
- Test substance identified as: FAT 31'016/I
- Appearance: solid
- Storage: room temperature (20-24 °C)
Oxygen conditions:
aerobic
Inoculum or test system:
other: Mixture of polyvalent bacteria collected on 9.11.82 from the sewage treatment plant (ARA Rhein)
Details on inoculum:
None
Duration of test (contact time):
28 d
Initial conc.:
40 mg/L
Based on:
DOC
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
TEST CONDITIONS
- Test temperature: 22 ± 1 °C
- Lighting: indirect daylight

TEST SYSTEM
- Exposure vessels: 500 ml Erlenmeyer flasks
- Water: According to the specification of the OECD Method 301 A
Reference substance:
other: glucose
Key result
Parameter:
% degradation (DOC removal)
Value:
0
Sampling time:
28 d

Results with reference substance:

Biodegradation corrected by the blank control and measured as DOC (mg/L) is for reference substance was calculated to be 94 % on 28th day.

Bacterial activity:

No inhibition of the activity of the bacteria was found.

Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
FAT 31016/I was concluded to have no biodegradation under test conditions.
Executive summary:

Biodegradation potential of the test substance FAT 31016/I was investigated in a study conducted according to the modified AFNOR test, Guideline OECD 301 A, May 12, 1981. Mixture of polyvalent bacteria collected from the sewage treatment plant (ARA Rhein) was used as test organism for inoculation. The level of biodegradation was calculated as the percentage elimination of the organic carbon. Initial test concentration was determined to be 40 mg/L DOC (nominal). The exposure was carried out for the duration of 28 days. However, 0% biodegradation was observed for FAT 31016/A, however for the reference substance glucose, the biodegradation was calculated to be 94 %. Hence, FAT 31016/I can be concluded to have no biodegradation under test conditions.

Endpoint:
biodegradation in water: screening test, other
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1993
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
None
Qualifier:
according to
Guideline:
ISO 5815 (Water quality - Determination of Biochemical Oxygen Demand after 5 Days (BOD5) - Dilution and Seeding Method)
Deviations:
yes
Remarks:
Aerate over night after addition of the inoculum
GLP compliance:
yes
Specific details on test material used for the study:
- Code number: FAT 31016/P
- En nr.: 414154.32
- Purity: 95 %
- Appearance: solid
- Solubility: 40 g/L (in water)
- Storage: room temperature
- Expiration date: 07/98
Oxygen conditions:
aerobic
Inoculum or test system:
other: Seeding water of an urban waste water
Details on inoculum:
None
Duration of test (contact time):
5 d
Initial conc.:
> 6 - < 800 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
TEST CONDITIONS
- Test concentrations : about 6 to 800 mg test substance / litre dilution water
- Temperature : 20 ± 1 °C
- Duration: 5 days
- Estimation Technique: O2 - Determination measured with an oxygen sensitive electrode system : SYLAND (SAV : 00 107)
- Suppression of nitrification : without suppression of nitrification
Reference substance:
other: Glucose
Remarks:
(Merck Nr. 8337)
Remarks on result:
not measured/tested
Key result
Parameter:
BOD5
Value:
0 mg O2/g test mat.
Results with reference substance:
The BOD5 of the reference substance is : 182 mg O2/I BOD5

None

Validity criteria fulfilled:
yes
Interpretation of results:
other: BOD5 was determined
Conclusions:
The BOD5 of FAT- 31'016/P was 0 mg 02/g.
Executive summary:

The biochemical oxygen demand of FAT - 31'016/P is determined by measuring the dissolved oxygen concentration before and after 5 days incubation at 20°C in different test concentrations diluted in water saturated with dissolved oxygen that contains a seed of microorganisms. The test was carried out according to ISO 5815 Second Edition 1989-08-01 (E) corresponding to92/96/EEC C.5. The test substance was tested in concentrations from about 6 to 800 mg/l. The reference substance D(+)-Glucose / L-Glutamic acid was tested in a concentration of 20 ml/I. The BOD5 of FAT- 31'016/P was 0 mg O2/g, while the BOD5 of the reference substance was 182 mg O2/l.

Endpoint:
biodegradation in water: screening test, other
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1993
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
other: DEV DIN 38'409 - 41-1 (1980)
Deviations:
yes
Remarks:
Mean of 2 determinations instead of 3
GLP compliance:
yes
Specific details on test material used for the study:
- Code number: FAT 31016/P
- En nr.: 414154.32
- Purity: 95 %
- Appearance: solid
- Solubility: 40 g/L (in water)
- Storage: room temperature
- Expiration date: 07/98
Oxygen conditions:
not specified
Inoculum or test system:
not specified
Details on inoculum:
None
Duration of test (contact time):
2 h
Details on study design:
- Sample weight: 2.5 mg of the test substance
- Temperature: 148 ± 3 °C
- Duration: 2 hours
- Estimation Technique: Titration (Potentiometric), METROHM Titroprozessor 670 (SAV : 00 111)
Reference substance:
other: Potassium hydrogen phthalate solution (Merck-Nr. 11769)
Remarks on result:
not measured/tested
Key result
Parameter:
COD
Value:
1 427 mg O2/g test mat.
Results with reference substance:
The COD of Potassium hydrogen phthalate (Validity: 200 ± 8 mg O2/I) was 198 mg O2/l.

None

Validity criteria fulfilled:
yes
Interpretation of results:
other: COD determined
Conclusions:
The COD of FAT- 31'016/P was 1427 mg O2/g.
Executive summary:

A predeterminated amount of the test substance FAT - 31'016/P dissolved in water is oxidized by potassium dichromate in a strong sulphuric acid medium with silver sulphate as a catalyst under reflux for 2 hours. The residual dichromate is determined by titration with standardized ferrous ammonium sulphate. The test was carried out according to DEV DIN 38'409 - H 41-1 (1980) corresponding to 92/96/EEC C.6. The test substance was tested with a sample weight of 2.5 mg. The reference substance Potassium hydrogen phthalate solution was tested in a concentration of 20 ml/I. The COD of FAT- 31'016/P was 1427 mg O2/g, while that of the reference substance was 198 mg O2/I

Endpoint:
biodegradation in water: inherent biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1994
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
None
Qualifier:
according to
Guideline:
OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
Version / remarks:
OECD Guideline for Testing of Chemicals, No. 302B, Paris 1981
Deviations:
yes
Remarks:
The sludge concentration in the test was 1040 mg/l instead of 200 -1000 mg/l as recommended in the guidelines.
GLP compliance:
yes
Specific details on test material used for the study:
- Code number: FAT 31016/P
- En nr.: 414154.32
- Purity: 95 %
- Appearance: solid
- Solubility: 40 g/L (in water)
- Storage: room temperature
- Expiration date: 07/98
Oxygen conditions:
aerobic
Inoculum or test system:
other: Sludge of a biological sewage treatment plant (ARA-Therwil) collected on 04.01.94
Details on inoculum:
None
Duration of test (contact time):
28 d
Initial conc.:
25 mg/L
Based on:
DOC
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
TEST CONDITIONS
- Test temperature: 22.0 ± 3°C (room temperature)
- Test duration: 2/8 days
- Lighting: indirect daylight
- Estimation Technique: TOC/DOC Analyzer/ SHIMADZU TOC-500
- Amount of substance (conc.): 50 mg/l
- Sludge (conc.): 1040 mg/l
Reference substance:
diethylene glycol
Preliminary study:
Mean value of preliminary tests: 27.0 mg/l
Key result
Parameter:
% degradation (DOC removal)
Value:
55.2
Sampling time:
28 d
Details on results:
Adsorption after 3 hours: 26.7 %

Results with reference substance:

The bioelimination, corrected by the blank control and measured as DOC (mg/l) is: 98.8 % by 16th day.

Validity criteria fulfilled:
yes
Interpretation of results:
inherently biodegradable, fulfilling specific criteria
Remarks:
inherent, primary biodegradability.
Conclusions:
FAT 31016/P was concluded to have potential for inherent, primary biodegradability.
Executive summary:

The inherent biodegradability of FAT - 31'016/P was determined in a 28 days Zahn-Wellens - Test according to the OECD Guideline for Testing of Chemicals, No. 302B, Paris 1981 and the EEC-Directive, L133 (pp. 99-105) Volume 31, May 88. The test substance was tested in concentration of 25 mg/l DOC. The reference substance Diethylenglycol was also tested in a concentration of 150 mg/l DOC. The bioelimination of the test substance FAT - 31'016/P after 28 days was determined to be 55.2 %, while the bioelimination of the reference substance after 16 days was 98.8 %. Hence, FAT 31016/P was concluded to have potential for inherent, primary biodegradability.

Description of key information

The substance is not readily biodegradable, however, possesses the potential for inherent, primary biodegradability.

Key value for chemical safety assessment

Biodegradation in water:
inherently biodegradable, fulfilling specific criteria

Additional information

FAT 31016/I was concluded to have no biodegradation under the test conditions in a ready biodegradability test conducted in accordance with AFNOR test, OECD Guideline 301A.

The bioelimination of the test substance FAT - 31'016/P after 28 days was determined to be 55.2 %, in a modified Zahn-Wellen's test conducted according to OECD Guideline 302B. Hence, FAT 31016/P was concluded to have potential for inherent, primary biodegradability.

In the separate studies investigating BOD5 and COD, the BOD5 of FAT 31016/P was determined to be 0 mg 02/g, while the COD was 1427 mg O2/g. Hence the BOD5/COD equals 0, hence indicating substance is not readily biodegradable. For FAT 31016/I, BOD5/COD ratio was again 0/1440 = 0, further supporting the conclusion that the substance is not readily biodegradable.

Hence, taking into account the substance can be concluded to be not readily biodegradable, however, to possess the potential for inherent, primary biodegradability.