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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1983
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
None
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Version / remarks:
Oct. 1980
Deviations:
no
GLP compliance:
no
Specific details on test material used for the study:
- Test Substance: FAT 31'016/i
- Appearance: solid
- Storage: room temperature (20 - 24°C)
Analytical monitoring:
yes
Vehicle:
not specified
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
- Weight: 0.29 g
- Length: 29.7 mm
- Age: 4 months
- Acclimation: 25 days in dechlorinated tap water
- Supplier: West-Aquarium, Bad Lauterberg, BRD
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Test temperature:
23 +/- 2°C
pH:
8.2 - 8.4
Dissolved oxygen:
6.1 - 7.3 O2 (mg/l)
Nominal and measured concentrations:
1.0, 1.78, 3.2, 5.6, 10.0, 17.8 and 32 mg/L (nominal)
Details on test conditions:
TEST SYSTEM
- Test vessel: 3 litre glass aquaria
- Aeration: slowly aerated
- Size: 10 fish per concentration and control
- Lighting: 12 hours light/12 hours darkness; yellow light (TL 16/40 W)

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : pH and O2, every 24 h intervals

TEST CONCENTRATIONS
Range finding study
- Test concentrations: A pretest was carried out at 1; 3; 10; 30; 100 ppm
- Results used to determine the conditions for the definitive study: In the pretest the approximate LC50 was determined to be 4 ppm.
Reference substance (positive control):
no
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
10.8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
No mortality was observed with the test concentrations 1.0, 1.78 and 3.2 mg/L. While 10, 30, 90 and 100% mortality was recorded at the test concentrations 5.6, 10.0, 17.8 and 32 mg/L respectively.
Reported statistics and error estimates:
LC50 -values and confidence limit are calculated with the Probit- Method (after E. Weber Grundriss der biologischen Statistik) .

None

Validity criteria fulfilled:
not specified
Conclusions:
The 96 h LC50 was calculated to be 10.8 mg/L.
Executive summary:

A study was conducted to evaluate the short term toxicity potential of FAT 31016/I to fish. The study was performed in accordance with the OECD Testguideline No. 203 (Oct. 1980). Based on the results obtained in the pretest the test concentrations were chosen as follows: 1,0; 1,78; 3,2; 5,6; 10; 17,8; 32 mg/L and control. 10 fish each were exposed at each concentration and observed for 96 hours. No mortality was observed with the test concentrations 1.0, 1.78 and 3.2 mg/L. While 10, 30, 90 and 100% mortality was recorded at the test concentrations 5.6, 10.0, 17.8 and 32 mg/L respectively. Hence, the LC50 was calculated to be 10.8 mg/L.

Description of key information

The LC50 to fish was determined to be 10.8 mg/L.

Key value for chemical safety assessment

LC50 for freshwater fish:
10.8 mg/L

Additional information

A study was conducted to evaluate the short term toxicity potential of FAT 31016/I to fish. The study was performed in accordance with the OECD Testguideline No. 203 (Oct. 1980). Based on the results obtained in the pretest the test concentrations were chosen as follows: 1,0; 1,78; 3,2; 5,6; 10; 17,8; 32 mg/L and control. 10 fish each were exposed at each concentration and observed for 96 hours. No mortality was observed with the test concentrations 1.0, 1.78 and 3.2 mg/L. While 10, 30, 90 and 100% mortality was recorded at the test concentrations 5.6, 10.0, 17.8 and 32 mg/L respectively. Hence, the LC50 was calculated to be 10.8 mg/L.