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EC number: 483-360-5
CAS number: 114435-02-8
Respiration rate at 30 min incubation
Respiration rate at 3h incubation
Inhibition at 30 min incubation
Inhibition at 3h incubation
The objective of this study was to determine the effect of F1EC
(MONOFLUOROETHYLENE CARBONATE) on activated sludge by measuring the
respiration rate under defined conditions in the presence of different
concentrations of the test substance.
3,5-Dichlorophenol was used as reference substance to evaluate the
sensitivity of the activated sludge. The test was conducted according to
OECD Guideline 209 (1984) and according to the OECD GLP guidelines
The respiration rate of an activated sludge suspension (4.0 g dry
weight/L) fed with a standard amount of synthetic wastewater was
measured after a contact time with the test substance of 30 minutes and
3 hours. The respiration rate is defined as the oxygen consumption of a
given concentration of activated sludge expressed as mg oxygen per liter
per minute. The study was carried out on one day on which both
3,5-dichlorophenol and F1EC were tested.
Two controls (replicate A and B) were included. As the difference
between the two controls was < 15%, the test can be considered as valid.
The reference test was conducted at concentrations of 5.0, 10 and 20 mg
3,5-dichlorophenol /L. The E(I)C50 value was estimated to be 5.5 mg/l
after 30 min and 3h of incubation. As the E(I)C50 value after 3 hours is
in the range of 5 to 30 mg/l it can be concluded that the sensitivity of
the activated sludge was sufficient.
The test with F1EC was conducted at concentrations of 5.0; 10; 20; 40;
80 and 160 mg/l. At the highest test concentration of 160 mg/L the
respiration inhibition was 24 and 19%, respectively after 30 minutes and
3 hours incubation. The E(I)C50 value after 30 minutes and 3 hours of
incubation was > 160 mg/L.
The 3 h IC50 of the substance to activated sludge were determined to be
above 160 mg/L and the NOEC 80 mg/L. The NOEC is used as the key value
for chemical safety assessment as the EC50 was determined to be greater
than the highest test concentration.
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