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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Description of key information

In this 48-hour acute toxicity test on Daphnia magna, the obtained results showed that the test item Methyl-isobutyl-ketone-peroxide (MIKP) had significant toxic effects on the mobility of Daphnia. The 48-h EC50 value was determined to be 4.48 mg/L. The 48-h NOEC was determined to be 2.00 mg/L and the 48-h LOEC was determined to be 5.11 mg/L. The results are based on measured test item concentrations.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
4.48 mg/L

Additional information

The influence of the test item Methyl-isobutyl-ketone-peroxide (MIKP) on the mobility and survival of Daphnia magna was determined in an acute toxicity study according to OECD guideline 202 and EU method C.2. 
Young Daphnia were exposed to aqueous test medium containing the test item at concentrations of 0.0256, 0.064, 0.16, 0.4 and 1.0 mg/L. The percentage of Daphnia being no longer capable of swimming at the end of the test or being dead was recorded for each test concentration. As just 5% immobility was observed in the test concentrations of 0.064 and 1 mg/L in this test the toxicity of test item was further investigated at higher concentrations of 2.5, 6.25 and 15.6 mg/L in a complementary experiment to determine the EC50 value.
The measured concentrations deviated more than 20 % from the nominal during the experiment therefore the geometric mean of the concentrations measured at the start and end of the water renewal periods were calculated to determine exposure concentrations. The corresponding calculated geometric mean test item concentrations were: 0.03, 0.04, 0.09, 0.21, 0.36, 2.00, 5.11 and 14.04 mg/L.
In this 48-hour acute toxicity test on Daphnia magna, the obtained results showed that the test item had significant toxic effects on the mobility of Daphnia. The 48-h EC50 value was determined to be 4.48 mg/L. The 48-h NOEC was determined to be 2.00 mg/L and the 48-h LOEC was determined to be 5.11 mg/L.