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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

In this 96-hour semi-static acute toxicity test on Zebrafish (Brachydanio rerio) the test item Methyl-isobutyl-ketone-peroxide (MIKP) had significant toxic effects on fish. The 96-h LC50 value was determined to be 1.89 mg/L. The 96-h NOEC and LOEC values were determined to be 1.38 mg/L and 2.62 mg/L, respectively. The results are based on measured test item concentrations.

Key value for chemical safety assessment

LC50 for freshwater fish:
1.89 mg/L

Additional information

The influence of the test item Methyl-isobutyl-ketone-peroxide (MIKP) to zebrafish (Brachydanio rerio) was evaluated in a short-term toxicity test according to OECD guideline 203 and EU method C.1. Young Zebrafish were exposed to aqueous test media containing the test item for 96 hours at a range of concentration in a semi-static test system (daily renewal periods). Based on the results of the preliminary experiment, nominal concentrations of 0.43, 0.94, 2.07, 4.55 and 10.00 mg/L were investigated in the main study. The corresponding measured test item concentrations (calculated as the geometric mean of the measured start and end values of each renewal periods) were: 0.26, 0.52, 1.38, 2.62 and 7.63 mg/L. The measured concentrations of the test item were in the range of 63 – 103 % of the nominal at the start and 30 – 70 % at the end of the renewal periods. Thus, all biological results are based on the measured geometric mean concentrations.

In this 96-hour semi-static acute toxicity test on Zebrafish the test item had significant toxic effects on fish. The 96-h LC50 value was determined to be 1.89 mg/L. The 96-h NOEC and LOEC vaues were was determined to be 1.38 mg/L and 2.62 mg/L, respectively.