Registration Dossier
Registration Dossier
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EC number: 219-228-9 | CAS number: 2390-54-7
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2-[4-(dimethylamino)phenyl]-3,6-dimethylbenzothiazolium chloride
- EC Number:
- 219-228-9
- EC Name:
- 2-[4-(dimethylamino)phenyl]-3,6-dimethylbenzothiazolium chloride
- Cas Number:
- 2390-54-7
- Molecular formula:
- C17H19N2S.Cl
- IUPAC Name:
- 2-[4-(dimethylamino)phenyl]-3,6-dimethylbenzothiazolium chloride
- Test material form:
- solid: particulate/powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- yes
- Remarks:
- ISO medium
Test organisms
- Test organisms (species):
- Daphnia magna
Study design
- Test type:
- static
- Water media type:
- other: ISO medium
- Total exposure duration:
- 48 h
Test conditions
- pH:
- The pH of aerated water was in the range of 6 - 9.
- Dissolved oxygen:
- Rhe dissolved oxygen was ≥ 3 mg/L.
- Nominal and measured concentrations:
- A static dose response test was conducted at nominal concentrations of 4.27 - 9.39 - 20.66 - 45.45 - 100.00 μg/L of test item in ISO medium, with an untreated group (without test item).
- Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- 8.31 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 19.7 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 4.27 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 9.39 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The acute immobilisation test was performed to assess the effects of test item Basic Yellow 1 on Daphnia magna after 48 hours of exposure under static conditions.
20 daphnids, less than 24 hours old, were exposed to 4.27 - 9.39 - 20.66 - 45.45 - 100.00 μg/L of test item in medium.
Moreover an untreated group (without test item) was be run in addition to the treatment groups.
The assessment of the effects was based on the nominal concentrations of test item and active ingredient Thioflavine.
After 48 hours, the endpoints based on the test item nominal concentrations were estimated to be the following:
EC10 8.31 μg/L; EC20 11.17 μg/L; EC50 19.70 μg/L;
LOEC: 9.39 μg/L;
NOEC: 4.27 μg/L.
After 48 hours, the endpoints based on the nominal concentrations of active ingredient Thioflavine were estimated to be the following:
EC10 6.73 μg a.i./L; EC20 9.04 μg a.i./L; EC50 15.95 μg a.i./L;
LOEC: 7.60 μg a.i./L;
NOEC: 3.46 μg a.i./L.
No other effects were observed on the exposed organisms. - Executive summary:
The acute immobilisation test was performed to assess the effects of test item Basic Yellow 1 on Daphnia magna after 48 hours of exposure under static conditions.
20 daphnids, less than 24 hours old, were exposed to 4.27 - 9.39 - 20.66 - 45.45 - 100.00 μg/L of test item in medium.
Moreover an untreated group (without test item) was be run in addition to the treatment groups.
The assessment of the effects was based on the nominal concentrations of test item and active ingredient Thioflavine.
After 48 hours, the endpoints based on the test item nominal concentrations were estimated to be the following:
EC10 8.31 μg/L; EC20 11.17 μg/L; EC50 19.70 μg/L;
LOEC: 9.39 μg/L;
NOEC: 4.27 μg/L.
After 48 hours, the endpoints based on the nominal concentrations of active ingredient Thioflavine were estimated to be the following:
EC10 6.73 μg a.i./L; EC20 9.04 μg a.i./L; EC50 15.95 μg a.i./L;
LOEC: 7.60 μg a.i./L;
NOEC: 3.46 μg a.i./L.
No other effects were observed on the exposed organisms.
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