Cyclohexylbenzene

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1953

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Old not-GLP study not according to international guideline.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1953

Report date:

1953

Materials and methods

Principles of method if other than guideline:

The intraperitoneal toxicity was found by the introduction of measured single doses into the peritoneal cavity of Sprague Dawley strain rats by means of hypodermic syringe and number 23 gauge needle. Frequent observations were made for outward signs of toxicity and all animals receiving a lethal dose were autopsied to make sure that each injection was properly made. The viscera was examined microscopically.

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Cyclohexylbenzene

EC Number:

212-572-0

EC Name:

Cyclohexylbenzene

Cas Number:

827-52-1

Molecular formula:

C12H16

IUPAC Name:

cyclohexylbenzene

Details on test material:

- Name of test material (as cited in study report): phenylcyclohexane

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

unchanged (no vehicle)

Control animals:

no

Results and discussion

Effect levels

Effect levels 1

Dose descriptor:

LD0

Effect level:

3.4 mL/kg bw

Based on:

test mat.

Effect levels 2

Dose descriptor:

LD50

Effect level:

3.7 mL/kg bw

Based on:

test mat.

Effect levels 3

Dose descriptor:

LD100

Effect level:

3.9 mL/kg bw

Based on:

test mat.

Any other information on results incl. tables

Sex	Weight (g)	Dose (ml/kg)	Weight change 5 days after dosing (%)	Fate
female	195	0.5	+3.5	survived
male	235	1.0	+2.0	survived
male	235	1.5	0.0	survived
female	210	2.0	0.0	survived
female	250	3.0	-6.0	survived
male	230	3.4	-4.5	survived
female	215	3.7		died - 120 hrs
male	240	3.7	-7.0	survived
male	225	3.9		died - 48 hrs
female	195	4.3		died - 72 hrs
female	220	5.0		died - 48 hrs
male	260	7.0		died - 54 hrs

Applicant's summary and conclusion

Conclusions:

The minimum lethal intraperitoneal dose was 3.4 to 3.7 ml/kg.

Executive summary:

The lethal dose was found to be 3.4 to 3.7 ml/kg with survival time ranging from 48 to 120 hrs. The animals became restless and lost appetite at injection levels of 1.0 ml/kg and above.

At autopsy, there was unabsorbed phenylcyclohexane in the peritoneal cavity colored somewhat by blood from surrounding tissue. Irritation was general throughout the cavity.