Registration Dossier
Registration Dossier
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EC number: 206-743-9 | CAS number: 372-09-8
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study, however the purity of the test substance was < 80 %
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Cyanoacetic acid
- EC Number:
- 206-743-9
- EC Name:
- Cyanoacetic acid
- Cas Number:
- 372-09-8
- Molecular formula:
- C3H3NO2
- IUPAC Name:
- cyanoacetic acid
- Details on test material:
- - Name of test material (as cited in study report): Cyanoacetic acid roh 70 %
- Physical state: liquid
- Analytical purity: 70.7 %,
- Lot/batch No.: 001V28AX10
- Composition of test material, percentage of components: 70.7 % Cyanoacetic acid, 21.5 % water
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- Municipal activated sludge from laboratory wastewater treatment plant fed with municipal sewage.
- Pretreatment: The inoculum was washed and brought to a concentration of 5 g/l dry substance, then aerated during the night. 50 ml were added to a total volume of 250 ml to obtain a concentration of 1 g/l dry substance in the test.
- Initial biomass concentration: 1 g/l
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 30 min
Test conditions
- Test temperature:
- 20 +/- 2 °C
- pH:
- before correction: 6.7 - 6.8
after correction: 7.6 - 7.8 - Dissolved oxygen:
- > 6.5 mg/l
- Nominal and measured concentrations:
- Nominal test concentration (mg/l):
248,
496,
1000,
control - Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer-vessel (nominal volume 250 ml)
- Fill volume: 250 ml
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: 8 ml/vessel 100-fold concentrated OECD medium
OTHER TEST CONDITIONS
- Adjustment of pH: yes
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 30 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- ca. 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 30 min
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
