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EC number: 206-992-3 | CAS number: 420-04-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1988
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1988
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: U.S. EPA 40 CFR Part 158.81.2
- Deviations:
- not applicable
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Cyanamide
- EC Number:
- 206-992-3
- EC Name:
- Cyanamide
- Cas Number:
- 420-04-2
- Molecular formula:
- CH2N2
- IUPAC Name:
- cyanamide
- Test material form:
- other: aqueous solution
- Details on test material:
- - Name of test material: Cyanamid L500 (50 % aqueous solution)
- Purity: 53 % (w/v)
- Appearance: Clear colourless liquid
- Lot/batch No.: 07/07/87
Constituent 1
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- The New Zealand White Rabbits were provided by Hazelton Research Products Inc., Denver Pennsylvania.
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- unchanged (no vehicle)
- Details on dermal exposure:
- The test substance was applied dermally to the shaved intact skin (approximately 10 % of the total body surface) of the test animals. The liquid test material was held in contact with the skin for a 24 hour period with impervious rubber damming.
- Duration of exposure:
- 24 h
- Doses:
- 530, 1060 and 2120 mg/kg bw of pure active substance Cyanamide, corresponding to 1.0, 2.0 and 4.0 mL/kg bw of 50 % aqueous Hydrogen cyanamide solution.
- No. of animals per sex per dose:
- 5 animals per dose per sex
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight.
Results and discussion
Effect levelsopen allclose all
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 901 mg/kg bw
- Based on:
- act. ingr.
- Remarks on result:
- other: Corresponding to 1.7 mL/kg bw of the 50 % aqueous Hydrogen cyanamide solution.
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 742 mg/kg bw
- Based on:
- act. ingr.
- Remarks on result:
- other: Corresponding to 1.4 mL/kg bw of the 50 % aqueous Hydrogen cyanamide solution.
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 848 mg/kg bw
- Based on:
- act. ingr.
- Remarks on result:
- other: Corresponding to 1.6 mL/kg bw of the 50 % aqueous Hydrogen cyanamide solution.
- Mortality:
- Mortality was observed in all dose groups, especially at mid (1060 mg/kg bw ) and high dose (2120 mg/kg bw) levels and occurred between day 1 and day 2 after treatment. No clinical observations were noted at the mid dose as all animals died on day 1.
- Clinical signs:
- other: Clinical signs of toxicology included tremors, ataxia, anorexia, depression and were observed at the low and high dose group. No signs of dermal irritations were noted in the low dose group, whereas all surviving animals of the high dose group showed a we
- Gross pathology:
- In rabbits that survived no pathologic changes at necropsy were observed. In rabbits found dead during the observation period changes mainly concerning the lung were observed (dark red lungs or dark red lung areas). In one animal of the high dose group a pale liver was noted.
- Other findings:
- No other findings
Any other information on results incl. tables
Table 1: Mortality in the acute dermal toxicity study in White rabbits with cyanamide
Males | Females | ||||
Dose | Mortality | Time of death | Dose | Mortality | Time of death |
530 mg/kg bw | 0/5 | - | 530 mg /kg bw | 1/5 | Day 1 |
1060 mg/kg bw | 5/5 | Day 1 and 2 | 1060 mg/kg bw | 5/5 | Day 2 |
2120 mg/kg bw | 4/5 | Day 2 | 2120 mg/kg bw | 5/5 | Day 2 |
Applicant's summary and conclusion
- Interpretation of results:
- Category 3 based on GHS criteria
- Conclusions:
- The acute dermal LD50 was 901 mg/kg bw in males, 742 mg/kg bw in females and 848 mg/kg bw combined for the sexes pure active substance cyanamide in rabbits (corresponding to about 1.6 mL/kg bw for the sexes combined, 1.7 mL/kg bw for males and 1.4 mL/kg bw for females of the 50 % aqueous Hydrogen cyanamide solution).
- Executive summary:
The test substance Cyanamid L500 was applied dermally to the shaved intact skin of five male and five female New Zealand White rabbits per dose group. 50 % w/w hydrogen cyanamide technical solution 1.0, 2.0 and 4.0 mL/kg bw (corresponding to 530, 1060 and 2120 mg/kg bw pure active substance cyanamide) were applied. The liquid test material was held in contact with the skin for a 24 hour period with impervious rubber damming. The animals were evaluated for treatment related effects on the day of dosing and for a subsequent 14-day observation period.
Clinical signs included tremors, ataxia, anorexia, depression were observed at the low and high dose group. No signs of dermal irritations were noted in the low dose group, whereas all surviving animals of the high dose group showed a well defined erythema and a very slightly oedema on day 1. All other animals died before primary dermal irritation scoring could be performed. In rabbits that survived no pathologic changes at necropsy were observed. In rabbits found dead during the observation period changes mainly concerning the lung were observed (dark red lungs or dark red lung areas). In one animal of the high dose group a pale liver was noted.
The acute dermal LD50 was 901 mg/kg bw in males, 742 mg/kg bw in females and 848 mg/kg bw combined for the sexes pure active substance cyanamide in rabbits (corresponding to about 1.6 mL/kg bw for the sexes combined, 1.7 mL/kg bw for males and 1.4 mL/kg bw for females of the 50 % aqueous Hydrogen cyanamide solution).
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