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EC number: 206-992-3 | CAS number: 420-04-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1983
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- ”Daphnia sp., 14 day Reproduction Test, including an Acute Immobilisation Test”, 1981
- Deviations:
- no
- Remarks:
- , however, the test concentrations are not quantified during the test duration. It is assumed that the test is valid without analytical data as cyanamide is known to be hydrolytically stable in water at the concentrations tested.
- GLP compliance:
- no
- Analytical monitoring:
- no
- Details on sampling:
- No sampling
- Vehicle:
- no
- Details on test solutions:
- Not indicated
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- - Unfed Daphnia magna, < 24 hours old
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- No post exposure observation period
- Hardness:
- Not indicated
- Test temperature:
- All chemical and physical parameters (dissolved oxygen concentration, pH, temperature) in the definitive test were within expected ranges.
- pH:
- All chemical and physical parameters (dissolved oxygen concentration, pH, temperature) in the definitive test were within expected ranges.
- Dissolved oxygen:
- All chemical and physical parameters (dissolved oxygen concentration, pH, temperature) in the definitive test were within expected ranges.
- Salinity:
- Freshwater
- Nominal and measured concentrations:
- Nominal concentrations of 1.8, 3.2, 5.6, 10.0, 18.0, 32.0, 56.0, 100.0, 180.0 and 320.0 mg of a 49 % (w/w) aqueous solution of cyanamide/L.
- Details on test conditions:
- Five daphnids were used per replicate. The test was performed with four replicates per treatment.
- Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 4.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Details on results:
- The test concentrations were not measured during the test. A summary of the findings is presented in Table A7.4.1.2/01-1. There were no test concentration related immobility or mortal effects below 5.6 mg of a 49 % (w/w) aqueous solution of cyanamide/L. The highest concentration causing no immobility or mortality was 3.2 mg/L and the lowest concentration causing 100 % immobility or mortality was 18.0 mg/L.
- Results with reference substance (positive control):
- No reference substance
- Reported statistics and error estimates:
- Not indicated
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The 48-hour EC50 in Daphnia magna was 6.5 mg of a 49 % (w/w) aqueous solution of cyanamide/L (equivalent to 3.2 mg as/L), based on immobilisation of the test animals. The 48-hour LC50 were 8.8 mg/L, based on mortality. The NOEC is estimated to be 3.2 mg/L (equivalent to 1.6 mg as/L). Since it is difficult to differentiate between immobile and dead daphnids, immobility is used as the relevant endpoint.
- Executive summary:
The acute toxicity of Alzodef (a 49 % (w/w) aqueous solution of cyanamide) to unfed Daphnia magna (< 24 hours old) was determined in an unaerated, static, 48-hour test. Treatments consisted of a diluted water control, and nominal concentrations of 1.8, 3.2, 5.6, 10.0, 18.0, 32.0, 56.0, 100.0, 180.0 and 320.0 mg of a 49 % (w/w) aqueous solution of cyanamide/L. Five daphnids were used per replicate. The test was performed with four replicates per treatment.
All chemical and physical parameters (dissolved oxygen concentration, pH, temperature) in the definitive test were within expected ranges. There were no test concentration related immobility or mortal effects below 5.6 mg of a 49 % (w/w) aqueous solution of cyanamide/L. The highest concentration causing no immobility or mortality was 3.2 mg/L and the lowest concentration causing 100 % immobility or mortality was 18.0 mg/L.
The 48-hour EC50 in Daphnia magna was 6.5 mg of a 49 % (w/w) aqueous solution of cyanamide/L (equivalent to 3.2 mg as/L), based on immobilisation of the test animals. The 48-hour LC50 were 8.8 mg/L, based on mortality. The NOEC is estimated to be 3.2 mg/L (equivalent to 1.6 mg as/L). Since it is difficult to differentiate between immobile and dead daphnids, immobility is used as the relevant endpoint.
Reference
Immobilisation of unfed Daphnia magna exposed to a 49 % (w/w) aqueous solution of cyanamide for 48 hours in an unaerated, static acute test:
Nominal concentration of a 49 % (w/w) aqueous solution of cyanamide (mg/L) | Mortality (No. dead/No. at test start) | Immobility (No. immobile/No. at test start | ||
| 24 hours | 48 hours | 24 hours | 48 hours |
Water Control | 0/20 | 0/20 | 0/20 | 0/20 |
1.8 | 0/20 | 0/20 | 0/20 | 0/20 |
3.2 | 0/20 | 0/20 | 0/20 | 0/20 |
5.6 | 1/20 | 3/20 | 1/20 | 6/20 |
10.0 | 4/20 | 11/20 | 7/20 | 18/20 |
18.0 | 13/20 | 20/20 | 15/20 | 20/20 |
32.0 | 11/20 | 20/20 | 11/20 | 20/20 |
56.0 | 12/20 | 20/20 | 12/20 | 20/20 |
100.0 | 12/20 | 20/20 | 13/20 | 20/20 |
180.0 | 19/20 | 20/20 | 20/20 | 20/20 |
320.0 | 20/20 | 20/20 | 20/20 | 20/20 |
Acute toxicity (48 h) of Cyanamide to daphnids – Summary of endpoints:
Endpoint
| Nominal concentration of 49 % (w/w) aqueous solution of cyanamide |
EC50 | 6.5 mg/L (3.2 mg as/L) |
LC50 | 8.8 mg/L (4.3 mg as/L) |
No observed effect concentration (NOEC) | 3.2 mg/L (1.6 mg as/L) |
Description of key information
The acute toxicity of the test substance cyanamide (Alzodef: 49 % (w/w) aqueous solution of cyanamide) to Daphnia magna was examined in one available study. In this static,
48 hours test the calculated EC50 was 3.2 mg of pure active ingredient (ai) cyanamide/L, NOEC of 1.6 mg ai/L and a LC50 of 4.3 mg ai/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 3.2 mg/L
Additional information
The acute toxicity of Alzodef (a 49 % (w/w) aqueous solution of cyanamide) to unfed Daphnia magna (< 24 hours old) was determined in an unaerated, static, 48-hour test. Treatments consisted of a diluted water control, and nominal concentrations of 1.8, 3.2, 5.6, 10.0, 18.0, 32.0, 56.0, 100.0, 180.0 and 320.0 mg of a 49 % (w/w) aqueous solution of cyanamide/L. Five daphnids were used per replicate. The test was performed with four replicates per treatment.
All chemical and physical parameters (dissolved oxygen concentration, pH, temperature) in the definitive test were within expected ranges. There were no test concentration related immobility or mortal effects below 5.6 mg of a 49 % (w/w) aqueous solution of cyanamide/L. The highest concentration causing no immobility or mortality was 3.2 mg/L and the lowest concentration causing 100 % immobility or mortality was 18.0 mg/L.
The 48-hour EC50 in Daphnia magna was 6.5 mg of a 49 % (w/w) aqueous solution of cyanamide/L (equivalent to 3.2 mg as/L), based on immobilisation of the test animals. The 48-hour LC50 were 8.8 mg/L, based on mortality. The NOEC is estimated to be 3.2 mg/L (equivalent to 1.6 mg as/L). Since it is difficult to differentiate between immobile and dead daphnids, immobility is used as the relevant endpoint.
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