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Diss Factsheets
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EC number: 206-992-3 | CAS number: 420-04-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Remarks:
- Growth inhibition
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1988
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- other: Guideline of the Umweltbundesamt ”Bewertung wassergefährdender Stoffe
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Details on sampling:
- No sampling
- Vehicle:
- no
- Details on test solutions:
- Not indicated
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- Not indicated
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 19 h
- Post exposure observation period:
- No post exposure observation period
- Hardness:
- Not indicated
- Test temperature:
- 25°C
- pH:
- Not indicated
- Dissolved oxygen:
- Not indicated
- Salinity:
- freshwater
- Nominal and measured concentrations:
- 100, 180, 320, 561 and 1002 mg/L of 49 % (w/w) aqueous solution of cyanamide. Measured values not available.
- Details on test conditions:
- In order to determine the percent growth inhibition, at the end of the study the optical density was measured in the nutrient solutions with the 49 % (w/w) aqueous solution of cyanamide and in the control solution without test substance.
- Reference substance (positive control):
- no
- Duration:
- 19 h
- Dose descriptor:
- other: TT: toxicity threshold (the concentration of the test substance at which its inhibitory action starts)
- Effect conc.:
- 157 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth inhibition
- Key result
- Duration:
- 19 h
- Dose descriptor:
- EC50
- Effect conc.:
- 283 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth inhibition
- Key result
- Duration:
- 19 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 88 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth inhibition
- Details on results:
- An inhibition of the growth of Pseudomonas putida was first observed at a concentration of 320 mg/L, where the growth was reduced for 10.3 % in comparison to the control. The highest dose (1002 mg/L) resulted in an inhibition of 90 % .
- Results with reference substance (positive control):
- No positive control
- Reported statistics and error estimates:
- Not indicated
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The toxicity threshold (19 hours) was found to be 320 mg product (49 % (w/w) aqueous solution of cyanamide/L), corresponding to 157 mg as/L. The EC50 (19 hours) was calculated to be 577 mg product/L, corresponding to 283 mg as/L. The NOEC was determined to be 180 mg product/L, corresponding to 88 mg as/L. Therefore, it can be concluded that cyanamide poses a very low risk to biological methods for sewage treatment. Due to its fast dissipation in soil and water, cyanamide is not expected to reach water processing facilities at high concentrations.
- Executive summary:
The influence of aqueous hydrogen cyanamide solution on the growth and degradation performance was conducted, using Pseudomonas putida as model organisms in fresh water. A 49 % (w/w) aqueous solution of cyanamide at concentrations of 100, 180, 320, 561 and 1002 mg/L and cultures of Pseudomonas putida were incubated for 19 hours at 25 °C. In order to determine the percent growth inhibition, at the end of the study the optical density was measured in the nutrient solutions with the 49 % (w/w) aqueous solution of cyanamide and in the control solution without test substance.
An inhibition of the growth of Pseudomonas putida was first observed at a concentration of 320 mg/L, where the growth was reduced for 10.3 % in comparison to the control. The highest dose (1002 mg/L) resulted in an inhibition of 90 %.
The toxicity threshold (19 hours) was found to be 320 mg product (49 % (w/w) aqueous solution of cyanamide/L), corresponding to 157 mg as/L. The EC50 (19 hours) was calculated to be 577 mg product/L, corresponding to 283 mg as/L. The NOEC was determined to be 180 mg product/L, corresponding to 88 mg as/L.
Therefore, it can be concluded that cyanamide poses a very low risk to biological methods for sewage treatment.
Due to its fast dissipation in soil and water, cyanamide is not expected to reach water processing facilities at high concentrations.
Reference
Effects of 49% (w/w) aqueous solution of cyanamide on biological methods for sewage treatment:
49% (w/w) aqueous solution of cyanamide (mg/L) |
Pure active ingredient cyanamide (mg/L) |
Growth inhibition (%) |
0 |
0 |
- |
100 |
49 |
0 |
180 |
88 |
0.6 |
320 |
157 |
10.3 |
561 |
275 |
69.1 |
1002 |
491 |
90.0 |
Description of key information
The toxicity of cyanamide to microorganisms was examined in one available study using Pseudomonas putida as model organisms in fresh water. The toxicity threshold (19 hours) was found to be 157 mg ai/L. The EC50 (19 hours) 283 mg as/L. The NOEC was determined to be 180 mg product/L, corresponding to 88 mg as/L. Therefore, it can be concluded that cyanamide poses a very low risk to biological methods for sewage treatment.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 283 mg/L
- EC10 or NOEC for microorganisms:
- 88 mg/L
Additional information
The toxicity of cyanamide (aqueous 49 % (w/w) hydrogen cyanamide solution) to microorganisms was examined in one available study using Pseudomonas putida as model organisms in fresh water. A 49 % (w/w) aqueous solution of cyanamide at concentrations of 100, 180, 320, 561 and 1002 mg/L were applied to cultures of Pseudomonas putida and incubated for 19 hours at 25 °C. In order to determine the percent growth inhibition, at the end of the study the optical density was measured in the nutrient solutions with the 49 % (w/w) aqueous solution of cyanamide and in the control solution without test substance. An inhibition of the growth of Pseudomonas putida was first observed at a concentration of 320 mg/L, where the growth was reduced for 10.3 % in comparison to the control. The highest dose (1002 mg/L) resulted in an inhibition of 90 %.
The toxicity threshold (19 hours) was found to be 320 mg product (49 % (w/w) aqueous solution of cyanamide/L), corresponding to 157 mg as/L. The EC50 (19 hours) was calculated to be 577 mg product/L, corresponding to 283 mg as/L. The NOEC was determined to be 180 mg product/L, corresponding to 88 mg as/L.
Therefore, it can be concluded that cyanamide poses a very low risk to biological methods for sewage treatment. Due to its fast dissipation in soil and water, cyanamide is not expected to reach water processing facilities at high concentrations.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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