Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 267-007-0 | CAS number: 67762-26-9 This substance is identified by SDA Substance Name: C14-C18 and C16-C18 unsaturated alkyl carboxylic acid methyl ester and SDA Reporting Number: 04-010-00.
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1999
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study not in GLP, purity of the sample has not been assessed, but the study is conducting following official guideline and scientifically valid in assessing the end point
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: ISO 10712
- Deviations:
- not specified
- GLP compliance:
- no
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- The sample is poor water soluble: the protocol ISO 147 has been followed. Saturated solutions have been prepared, the more concentrated at 10% (10g/100ml).
Starting from that, following dilution have been prepared: 8%, 1%, 0.1%, 0.01%, 0.001% - Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- -Age of the culture stock: 7 days
-Age of pre-colture: 5+/- h at 25°C - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 16 h
- Key result
- Duration:
- 16 h
- Dose descriptor:
- EC0
- Effect conc.:
- ca. 5 250 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: WSF: Water Soluble Fraction
- Basis for effect:
- growth inhibition
- Reported statistics and error estimates:
- Calculation mehtod: Trimmed Spearman-Karber method
- Conclusions:
- WSF EC0 16h Pseudonomas putida = 5250 mg/l
- Executive summary:
The fatty acid, rape oil, methyl ester has been tested for The inhibition of the growth of Pseudonomas Putida following ISO 10712 and ISO 147 for poorly soluble substances
Reference
| Concentration | Absorbtion 0h | Absorption 16h | Difference | % Inhibition |
| Control | 0.015 | 0.101 | 0.086 | - |
| WSF 8% | 0.015 | 0.088 | 0.073 | 15 |
| WSF 1% | 0.01 | 0.093 | 0.08 | 3 |
| WSF 0.1% | 0.01 | 0.10 | 0.089 | 0 |
| WSF 0.01% | 0.01 | 0.097 | 0.087 | 0 |
| WSF 0.001% | 0.01 | 0.099 | 0.089 | 0 |
Description of key information
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 5 250 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
