Diphenyl methylphosphonate

Biodegradation in soil

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in soil: simulation testing

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

Please see "Read-across Justification" section 13

Reason / purpose for cross-reference:

read-across source

Duration:

96 h

Remarks on result:

other: Publication about the C-P cleavage potential of Agrobacterium radiobacter (isolated from sewage sludge) in Methylphosphonic Acid (MPA), which is a metabolite of Diphenyl methylphosphonate (DPP).

Transformation products:

not measured

Details on transformation products:

No details available.

Evaporation of parent compound:

not measured

Volatile metabolites:

not measured

Residues:

not measured

Details on results:

Pi supported the most rapid growth of Agrobacterium radiobacter, the specific growth rate was determined as 0.26/h.
A. radiobacter executes more C-P bond cleavage than necessary to attain its Pi requirements. A greater gas production was observed with substrates that supports higher growth rates. The major gaseous product was Methane (CH4).
Furthermore it is reported, that several Escherichia coli laboratory strains grew on methylphosphonic acid, suggesting that C-P lyase activity is not a trait that is readily lost during laboratory culture.

Results with reference substance:

Many substances were investigated, but no specific reference substance. Growth was scored as a percentage of the final absorbance attained during the same period as the test substance by a Pi (inorganic phosphorous) control.

Conclusions:

Publication about the C-P cleavage potential of Agrobacterium radiobacter (isolated from sewage sludge) in Methylphosphonic Acid (MPA), which is a metabolite of Diphenyl methylphosphonate (DPP).

Executive summary:

Wackett et al. (1987) investigated the carbon - phosphorus (C-P) bond cleavage by Agrobacterium radiobacter in Methylphosphonic acid (MPA), which was found to be a metabolite of Diphenyl methylphosphonate (DPP). A. radiobacter was isolated from sewage sludge from a water treatment plant. After four successive enrichment cultures, bacteria were streaked onto nutrient agar plates. Strains were selected for taxonomic identification were examined by Gram staining and then inoculated into API-E and API-NFT test strips. The identification of the selected bacterium was confirmed by its uniquely positive result on 3-ketolactose indicator plates. Many substances were investigated, but no specific reference substance. Growth was scored as a percentage of the final absorbance attained during the same period as the test substance by a Pi (inorganic phosphorous) control. Pi supported the most rapid growth of A. radiobacter, the specific growth rate was determined as 0.26/h. The bacterium executes more C-P bond cleavage than necessary to attain its Pi requirements. A greater gas production was observed with substrates that supports higher growth rates. The major gaseous product was Methane (CH4). Furthermore it is reported, that several Escherichia coli laboratory strains grew on methylphosphonic acid, suggesting that C-P lyase activity is not a trait that is readily lost during laboratory culture.

Description of key information

Experimental result with MPA (Metabolite of DPP): C-P bond cleavage by Agrobacterium radiobacter: more bond cleavage is executed than necessary for Pi requirements. The major gaseous product was Methane.  

Additional information

Wackett et al. (1987) investigated the carbon - phosphorus (C-P) bond cleavage by Agrobacterium radiobacter in Methylphosphonic acid (MPA), which was found to be a metabolite of Diphenyl methylphosphonate (DPP). A. radiobacter was isolated from sewage sludge from a water treatment plant. After four successive enrichment cultures, bacteria were streaked onto nutrient agar plates. Strains were selected for taxonomic identification were examined by Gram staining and then inoculated into API-E and API-NFT test strips. The identification of the selected bacterium was confirmed by its uniquely positive result on 3-ketolactose indicator plates. Many substances were investigated, but no specific reference substance. Growth was scored as a percentage of the final absorbance attained during the same period as the test substance by a Pi (inorganic phosphorous) control. Pi supported the most rapid growth of A. radiobacter, the specific growth rate was determined as 0.26/h. The bacterium executes more C-P bond cleavage than necessary to attain its Pi requirements. A greater gas production was observed with substrates that supports higher growth rates. The major gaseous product was Methane (CH4). Furthermore it is reported, that several Escherichia coli laboratory strains grew on methylphosphonic acid, suggesting that C-P lyase activity is not a trait that is readily lost during laboratory culture.