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EC number: 905-562-9
CAS number: -
The lowest results is a 28 day NOEC of 16 mg/L for xylene isomers and a 24 hour IC50 of 96 mg/L for ethylbenzene.
Biodegradation tests were carried out on all three xylene isomers
following OECD 301F guidelines using a mixed inoculum of non-adapted
sludge and soil micro-organisms. In the toxicity control vessels,
containing the individual xylene isomers with the reference substance
sodium benzoate, none of the xylene isomers exhibited inhibitory effects
on the micro-organisms. The 28 day NOEC (nominal concentration) is
therefore 16 mg/L for m-xylene and 16.2 mg/L for o-xylene and p-xylene.
The studies by TNO (Oldersma 2004) and Volskay and Grady (1988) both
follow OECD 209. TNO (2004) used p-xylene as the test substance, Volskay
and Grady (1988) tested both o- and p-xylene. Each of the tests used a
single test concentration approximating the limit of solubility of the
test substance. The EC50 was not observed in any of the tests. The study
by TNO reports a 3 hour NOEC of 157mg/L. Volskay and Grady (1988) report
a 30 minute EC50 (respiration inhibition) of >175 mg/L for o-xylene and
>198 mg/L for p-xylene and therefore the EC50 are above the limit of
A non-guideline study by Blum and Speece
(1991) used ethylbenzene as the test substance and reported a 24 hour
IC50 for Nitrosomonas of 96 mg/L. This result is cited in the EU
RAR (ECB 2007) along with a 15 hour IC50 of 130 mg/L for the inhibition
of oxygen consumption of aerobic heterotrophs from the same study. The
EU RAR also reports results for Volskay and Grady (1990), Krebs (1991)
and earlier studies by Bringmann and Kuhn (1977, 1981) but, as more
reliable data were available, these were not considered to be the key
studies and so have not been included here.
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