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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP status not known, near guideline study, published in peer reviewed literature, minor restrictions in design and/or reporting but otherwise adequate for assessment.

Data source

Reference
Reference Type:
publication
Title:
The toxicological properties of hydrocarbon solvents
Author:
Hine CH, Zuidema HH
Year:
1970
Bibliographic source:
Industrial Medicine 39, 215-200.

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
EU Method B.2 (Acute Toxicity (Inhalation))
Principles of method if other than guideline:
Groups of 10 male rats were exposed to (unspecified) concentrations of C-8 aromatics for 4 hours followed by a 14 day observation period.
GLP compliance:
not specified
Test type:
standard acute method
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
C-8 aromatics (ortho, meta and para xylene, ethylbenzene composition not defined). Boiling range 138-141°C.

Test animals

Species:
rat
Strain:
Long-Evans
Sex:
male
Details on test animals and environmental conditions:
TEST ANIMALS
Body weight 150 - 300 g.

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
other: air
Details on inhalation exposure:
A 250 L chamber was used for the vapour exposures. Air entering the chamber was bubbled through the sample, heated to 25°C in a water bath in order to obtain an atmosphere as close to saturated as possible. Vapour concentration in the chamber was monitored by means of a Wilkens Aerograph GLC and compared with the vapour concentration obtained from a bottle known to contain vapours saturated at 25C. For graded concentrations less than saturated, the air flow entering the chamber was divided so that only part of it passed through the sample. Rats were exposed for four hours and ten rats were used for each exposure.
Analytical verification of test atmosphere concentrations:
yes
Duration of exposure:
4 h
Concentrations:
not stated
No. of animals per sex per dose:
10
Control animals:
not specified
Details on study design:
Animals were retained for a minimum of 14 days after exposure and mortality values were based on the number of animals which died during this interval.

Statistics:
LD50 values were determined by the method of Litchfield and Wilcoxon

Results and discussion

Effect levels
Sex:
male
Dose descriptor:
LC50
Effect level:
6 350 ppm
95% CL:
4 670 - 8 640
Exp. duration:
4 h
Remarks on result:
other: 27571 mg/m3
Mortality:
All deaths (number not reported) occurred during exposure.
Clinical signs:
Survivors were comatosed but recovered shortly following removal from the chamber.

Applicant's summary and conclusion

Interpretation of results:
not classified
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
The inhalation LC50 was 6350 ppm in the rat.

COMMENT TO TSC: conversion to mg/L required once phys-chem properties confirmed.
Executive summary:

Groups of male rats were exposed to (unspecified) concentrations of C-8 aromatics for 4 hours followed by a 14 day observation period. All exposures caused CNS depression (animals described as being in a comatose state), with an overall LC50 of 6350 ppm.