3-chlorophenyl isocyanate

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: No OECD Guideline defined.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

not specified

Remarks:

only 4 test strains used

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

3-chlorophenyl isocyanate

EC Number:

220-822-5

EC Name:

3-chlorophenyl isocyanate

Cas Number:

2909-38-8

Molecular formula:

C7H4ClNO

IUPAC Name:

1-chloro-3-isocyanatobenzene

Details on test material:

purity: 99.8%, clear liquid, molecular weight: 153.6

Method

Target gene:

Ames assay

Species / strain

Species / strain / cell type:

other: Salmonella typhimurium TA 98, TA 100, TA 1535, TA 1537

Metabolic activation:

with and without

Metabolic activation system:

S9

Test concentrations with justification for top dose:

up to 8 µg per plate

Controls

Untreated negative controls:

yes

Negative solvent / vehicle controls:

not specified

True negative controls:

yes

Positive controls:

yes

Positive control substance:

other: sodium azide, nitrofurantoin, 4-nitro-1.2-phenylene diamine and 2-aminoanthracene

Details on test system and experimental conditions:

IUCLID4 Type: Ames test

Results and discussion

Test results

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

not specified

Untreated negative controls validity:

valid

Positive controls validity:

valid

Remarks on result:

other: other: S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

Summary of the results with the test substance in the Salmonella/ Microsome Test

S9 mix	TA 1535	TA 100	TA 1537	TA 98
without	negative	negative	negative	negative
with	negative	negative	negative	negative

Applicant's summary and conclusion

Executive summary:

The test substance was investigated using the Salmonella/ microsome test for point mutagenic effects in doses up to 5000 µg per plate on four Salmonella typhimurium LT2 mutants. These comprised the histidine-auxotrophic strains TA 1535, TA 100, TA 1537 and TA 98.

Doses up to and including 8 µg per plate did not cause any bacteriotoxic effects: Total bacteria counts remained unchanged and no inhibition of growth was observed. At higher doses, the substance had a strong, strain-specific and S9 -dependent bacteriotoxic effect, so that this range could only be used to a limited extent up to 200 µg per plate (without S9 mix) and 1000 µg per plate (with S9 mix) for assessment purposes. Substance precipitation occured at 1000 µg per plate and above.

The positive controls sodium azide, nitrofurantoin, 4 -nitro-1,2 -phenylene diamine and 2 -aminoanthracene had a marked mutagenic effect, as was seen by a biologically relevant increase in mutant colonies compared to the corresponding negative controls.

Evidence of mutagenic activity of the test substance was not seen.

No biologically relevant increase in the mutant count, in comparison with the negative controls, was observed. Therefore the test substance was considered to be non-mutagenic without and with S9 mix in the Salmonella/ microsome test.