3-chlorophenyl isocyanate

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: OECD Guideline study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1991

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

GLP compliance:

yes

Test type:

other: Acute inhalation study in rats

Test material

Test material information

Constituent 1

Reference substance name:

3-chlorophenyl isocyanate

EC Number:

220-822-5

EC Name:

3-chlorophenyl isocyanate

Cas Number:

2909-38-8

Molecular formula:

C7H4ClNO

IUPAC Name:

1-chloro-3-isocyanatobenzene

Details on test material:

Purity: 99.8%, Molecular weight: 153.6 g/mol, liquid substance.

Test animals

Species:

rat

Strain:

other: Bor: WISW

Sex:

male/female

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

not specified

Vehicle:

not specified

Analytical verification of test atmosphere concentrations:

yes

Duration of exposure:

4 h

Concentrations:

8, 39, 77, 150, 181 mg/m³

No. of animals per sex per dose:

5

Control animals:

yes

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LC50

Effect level:

58 mg/m³ air

Effect levels 2

Sex:

female

Dose descriptor:

LC50

Effect level:

39 mg/m³ air

Effect levels 3

Sex:

male/female

Dose descriptor:

LC50

Effect level:

42 mg/m³ air

95% CL:

31 - 57

Exp. duration:

4 h

Any other information on results incl. tables

Results:

Animal No.	Concentration (mg/m³ air)		Toxicological result	Duration of symptom	Timepoint of death	Mortality (%)
	nominal	analytic
Rat, male
1	air-control		0/0/5*	--	--	0
2	8	2.1	0/0/5	--	--	0
3	39	8.4	0/5/5	4h-3d	--	0
4	77	39.3	1/5/5	4h-28d	10d	20
5	150	66.2	3/5/5	4h-28d	7d-12d	60
6	181	108.7	5/5/5	4h-11d	6d-11d	100
Approximative LC50 = 58 mg/m³ air.
Rat, female
1	air-control		0/0/5	--	--	0
2	8	2.1	0/0/5	--	--	0
3	39	8.4	0/3/5	4h-1d	--	0
4	77	39.3	3/5/5	4h-28d	8d-15d	60
5	150	66.2	5/5/5	4h-14d	7d-14d	100
6	181	108.7	5/5/5	4h-9d	1d-9d	100
Approximative LC 50 = 39 mg/m³ air.

LC50 (male/female together) = 42 mg/m³ air.

Confidencelevel (95%)= 31 -57 mg/m³ air.

* 1. Nr.: Number of died animals;

2. Nr.: Number of animals with symptoms;

3. Nr.: Number of animals used.

Symptoms:

Group 1 and 2: no effects observed.

Group 3: diminuated breathing, serous defluxion out of nose, sounds of coryza, and reddened rhinarium.

Group 4: unkempt fur, sounds of breathing, diminuated and hindered breathing, serous defluxion out of nose, bloody rhinarium, cyanosis, tachipnosis, sounds of coryza, atony, cachexia, diminuated motility.

Group 5: unkempt fur, sounds of breathing, decelerated and hindered breathing, serous defuxion out of nose, bloody rhinarium, sounds of coryza, cyanosis, atony, salivation, diminuated motility and cachexia.

Group 6: unkempt fur, palpebral fissure narrowed, sounds of coryza, dyspnea, sounds of breathing, diminuated and hindered breathing, serous fefluxion out of nose, bloody/ blood encrusted rhinarium, cyanosis, salivation, cachexia, diminuated motility and atony.

Applicant's summary and conclusion

Executive summary:

In an acute oral toxicity study in male and female rats according OECD guideline 403 the animals were exposed for 4h to following concentrations of test substance: 8, 39, 77, 150, 181 mg/m³. The observation period was 4 weeks.

The LC50 for male and female rats was 42 mg/m³ air (LC 50 male: 58 mg/m³air; LC 50 female: 39 mg/m³).