1,3,5-Triazine-2,4-diamine, N2-[(1R,2S)-2,3-dihydro-2,6-dimethyl-1H-inden-1-yl]-6-(1-fluoroethyl)-

Toxicity to soil microorganisms

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

toxicity to soil microorganisms

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 216 (Soil Microorganisms: Nitrogen Transformation Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Vehicle:

no

Details on preparation and application of test substrate:

AMENDMENT OF SOIL
- Type of organic substrate: Biologically active agricultural soil; loamy sand soil, pH 6.8, 1.43 % Corg, WHC: 41.03 g/100 g dry soil.


APPLICATION OF TEST SUBSTANCE TO SOIL
- Method: 150 g soil dry weight (soil d.w.) per test vessel was weighed. The soil was mixed with 0.5 % (i.e. 0.75 g/150g
soil d.w.) lucerne meal by means of a hand-stirrer.

Test organisms (inoculum):

soil

Total exposure duration:

28 d

Test temperature:

19.3 - 20.9 °C

Moisture:

Soil moisture: approx. 45 % of its maximum water holding capacity; water content: 17.77 - 18.10 g/100 g dry soil

Details on test conditions:

TEST SYSTEM
- Amount of soil: 10 g soil d.w.
- No. of replicates per concentration: 3
- No. of replicates per control:3



SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographical reference of sampling site (latitude, longitude): Germany, Sachsen, Canitz, Schlag 34/3, Wassergut Canitz
G PS-position: 12.694435960 degrees East and 51.403774567 degrees North
- Treatments with pesticides or fertilizers: none since 2003
- Accidental contamination: no
- Depth of sampling: 0.05 m
- Soil texture
- % sand: 51.4
- % silt: 37.7
- % clay: 10.8
- pH (in water): 6.8
- Maximum water holding capacity (in % dry weigth): 41.03
- Cation exchange capacity (cmol+/kg soil d.w.): 10.8

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Soil samples (10 g soil d.w. per replicate) were taken at intervals of 3 hours, 7, 14 and 28 days after application and the NH4-N, NO3-N and NO2-N content were determined.

Nominal and measured concentrations:

Application rates in the test:
150 g a.i./ha (equivalent to 159 g test item/ha)
750 g a.i./ha (equivalent to 794 g test item/ha)

Test concentrations:
0.21 mg test item/kg soil dry weight
1.06 mg test item/kg soil dry weight

Reference substance (positive control):

yes

Remarks:

Dinoterb

Duration:

28 d

Dose descriptor:

NOEC

Effect conc.:

>= 750 g/ha

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

nitrate formation rate

Duration:

28 d

Dose descriptor:

NOEC

Effect conc.:

>= 1.06 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

nitrate formation rate

Reported statistics and error estimates:

Statistical analysis (Student-t-test, 2-sided, p <= 0.05) revealed no significant differences for both tested concentrations compared to control 28 days after treatment.

CONCLUSION

The test substance caused no adverse effects (deviation from control <25 %, OECD 216) on the soil nitrogen transformation (measured as NO3-N production) at the end of the 28-day incubation period. The study was performed in a field soil at concentrations equivalent up to a field application rate of 750 g a.i./ha (equivalent to 794 g test item/ha).

Reference 2

Endpoint:

toxicity to soil microorganisms

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 217 (Soil Microorganisms: Carbon Transformation Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Vehicle:

no

Details on preparation and application of test substrate:

AMENDMENT OF SOIL
- Type of organic substrate: Biologically active agricultural soil; loamy sand soil, pH 6.8, 1.43 % Corg, WHC: 41.03 g/100 g dry soil.


APPLICATION OF TEST SUBSTANCE TO SOIL
- Method:

Test organisms (inoculum):

soil

Total exposure duration:

28 d

Test temperature:

19.3 - 20.9 °C

Moisture:

approx. 45 % of its maximum water holding capacity; water content: 18.13 - 18.36 g/100 g dry soil; pH 6.5 - 6.8.

Details on test conditions:

TEST SYSTEM
- Amount of soil: 10 g soil d.w.
- No. of replicates per concentration: 3
- No. of replicates per control:3

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographical reference of sampling site (latitude, longitude): Germany, Sachsen, Canitz, Schlag 34/3, Wassergut Canitz
G PS-position: 12.694435960 degrees East and 51.403774567 degrees North
- Treatments with pesticides or fertilizers: none since 2003
- Accidental contamination: no
- Depth of sampling: 0.05 m
- Soil texture
- % sand: 51.4
- % silt: 37.7
- % clay: 10.8
- pH (in water): 6.8
- Maximum water holding capacity (in % dry weigth): 41.03
- Cation exchange capacity (cmol+/kg soil d.w.): 10.8

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): The short-term respiration was determined on day 0 (after approximately 3 hours) and at intervals of 7, 14, and 28 days after application. A respirometer system was used to determine the O2-consumption over a
period of maximum 24 hours at different sampling intervals.

Nominal and measured concentrations:

Application rates of the study:
- Quartz meal only (Control)
- 150 g a.i./ha (equivalent to 159 g test item/ha)
- 750 g a.i./ha (equivalent to 794 g test item/ha)

Test concentrations:
0.21 mg test item/kg soil dry weight
1.06 mg test item/kg soil dry weight

Reference substance (positive control):

yes

Remarks:

Dinoterb

Duration:

28 d

Dose descriptor:

NOEC

Effect conc.:

>= 750 g/ha

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

respiration rate

Duration:

28 d

Dose descriptor:

NOEC

Effect conc.:

>= 1.06 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

respiration rate

CONCLUSION AE 1170437 tech. caused no adverse effects (deviation from control < 25 %, OECD 217) on the soil carbon transformation (measured as oxygen consumption) at the end of the 28-day incubation period. The study was performed in a field soil at concentrations equivalent up to a field application rate of 750 g a.i./ha (equivalent to 794 g test item/ha).

Description of key information

NOEC (28 d) >= 1.06 mg a.s./kg soil dw

Additional information

Two GLP studies are available. No adverse effects of the test substance on carbon transformation (OECD 217) and on nitrogen transformation (OECD 216) in soil were observed in both tests systems with concentrations of 0.21 mg test item/kg dry soil (corresponding to an application rate of 150 g a.s./ha = 159 g test item/ha) and 1.06 mg test item/kg dry soil (corresponding to an application rate of 750 g a.s./ha = 794 g test item/ha). Test concentrations related to a soil depth of 5 cm and a soil density of 1.5 g/cm³.

Therefore it can be concluded that the test substance caused no negative effects (deviation from control <25 %, OECD 216) on the soil nitrogen transformation (measured as NO3-N production) and also no negative effects (deviation from control <25 %, OECD 217) on the soil carbon transformation (measured as oxygen consumption) at the end of the 28-day incubation period.

A statistical analysis was performed (t-test) in order to derive the NOEC-value. Since no statistical difference resulted the NOEC (28 d) for both studies was derived to be >= 750 g a.s./ha corresponding to >=1.06 mg a.s./kg soil dw.