1,3,5-Triazine-2,4-diamine, N2-[(1R,2S)-2,3-dihydro-2,6-dimethyl-1H-inden-1-yl]-6-(1-fluoroethyl)-

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.5400 (Algal Toxicity, Tiers I and II) (January 2012)

Version / remarks:

, draft

Qualifier:

according to guideline

Guideline:

other: FIFRA Guideline 123-2 (1982)

Principles of method if other than guideline:

FIFRA Guideline 123-2 (1982), OPPTS Guideline 850.4500 (2006 draft), OECD Guideline 201 (2006). The afore mentioned guidelines were harmonized for various test parameters (i.e. temperature, light, etc.) to achieve optimal environmental conditions for the test organism. Scientific discretion was implemented where guideline parameters do not fully converge.

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Chemical name of vehicle: Acetone
- Concentration of vehicle in test medium: 0.1 ml/L

Test organisms (species):

Navicula pelliculosa

Details on test organisms:

TEST ORGANISM
- Common name: Diatom
- Source: In-house 3 day old batch culture held under test conditions, original optained from University of Texas (UTEX), Austin, Texas, USA, received September 13, 2005
- Age of inoculum: 3 days

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

24 ± 2.0 °C

pH:

7.2 to 9.5

Nominal and measured concentrations:

Nominal concentrations: 2, 20, 200 and 2000 μg a.i./L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: Borosilicate glass; sterile 250-mL Erlenmeyer flasks covered by inverted glass beakers.
- Initial cells density:
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- No. of vessels per vehicle control (replicates): 3

GROWTH MEDIUM
- Standard medium used: yes

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline


OTHER TEST CONDITIONS
- Photoperiod: 24 hours Light
- Light intensity and quality: Cool white fluorescents, 4400 to 4940 lux (measured range)


EFFECT PARAMETERS MEASURED:
- Determination of cell concentrations: Daily, Manual counts via light microscope and hemocytometer slide.

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

0.24 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

act. ingr.

Basis for effect:

growth rate

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

0.057 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

act. ingr.

Basis for effect:

growth rate

Observations:

No physical abnormalities were observed in the controls or treatment groups during the study.

Conclusions:

The 72 and 96-hour growth rates were calculated based on mean measured concentrations.

The 72 hour EC50 value for growth rate (ErC50) is 240 μg a.i./L with LOEC and NOEC values of 199 and 57.4 μg a.i./L, respectively. The 96-hour EC50 value for growth rate is 397 μg a.i./L with LOEC and NOEC values of 199 and 57.4 μg a.i./L, respectively.

Reference 2

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.5400 (Algal Toxicity, Tiers I and II) (January 2012)

Version / remarks:

(2006 draft)

Qualifier:

according to guideline

Guideline:

other: FIFRA Guideline 123-2 (1982)

Principles of method if other than guideline:

The afore mentioned guidelines were harmonized for various test parameters (i.e. temperature, light, etc.) to achieve optimal environmental conditions for the test organism. Scientific discretion was implemented where guideline parameters do not fully converge.

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Vehicle:

yes

Details on test solutions:

- Chemical name of vehicle: Acetone

Test organisms (species):

Anabaena flos-aquae

Details on test organisms:

TEST ORGANISM
- Common name: Blue Green Algae
- Source : Own culture, originally optained from University of Texas (UTEX), Austin, Texas, USA, received August 1, 2006

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

23.4 to 24.1 °C

pH:

7.3 to 9.7

Nominal and measured concentrations:

Nominal Test Concentrations: Control, Solvent Control, 13, 40, 127, 400, 1270, and 4000 μg a.i./L
Mean Measured Test Concentrations: Control (

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: Borosilicate glass, Sterile 250-mL Erlenmeyer flasks covered by inverted glass beakers; Fill volume of 100 ml
- Initial cells density: 1.0 x 10E4 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- No. of vessels per vehicle control (replicates): 3

GROWTH MEDIUM
- Standard medium used: yes

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline


OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 24 hour light
- Light intensity and quality: 1950 – 2400 lux (Cool white fluorescents)


EFFECT PARAMETERS MEASURED:
Cell Density Measurement Technique: Manual counts via light microscope and hemocytometer slide
Measurement Interval: Daily
Cellular Observations: Visual inspection via light microscope
Observation Interval: Daily

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

3.859 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

act. ingr.

Basis for effect:

growth rate

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

0.115 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

act. ingr.

Basis for effect:

growth rate

Observations:

No physical abnormalities were observed in the controls or treatment groups during the study.

Conclusions:

The 48 and 96-hour growth rates values were calculated based on mean measured concentrations. The 48 hour EC50 value for growth rate (ErC50) for the 48 to 96 hour study period is 3432 μg a.i./L with LOEC and NOEC values of 1294 and 406 μg a.i./L, respectively. The 96-hour ErC50 is 3859 μg a.i./L with LOEC and NOEC values of 406 and 115 μg a.i./L, respectively.

Reference 3

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.5400 (Algal Toxicity, Tiers I and II) (January 2012)

Version / remarks:

(2006 draft)

Qualifier:

according to guideline

Guideline:

other: FIFRA Guideline 123-2 (1982)

Principles of method if other than guideline:

The afore mentioned guidelines were harmonized for various test parameters (i.e. temperature, light, etc.) to achieve optimal environmental conditions for the test organism. Scientific discretion was implemented where guideline parameters do not fully converge.

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
see also materials and methods
- Chemical name of vehicle: Acetone

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM
- Common name: Green Algae
- Source : Own culture, originally optained from University of Texas (UTEX), Austin, Texas, USA

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

24 ± 2.0 °C

pH:

7.4 to 9.8

Nominal and measured concentrations:

Nominal Test Concentrations: Control, Solvent Control, 25, 50, 100, 200 and 400 μg a.i./L
Mean Measured Test Concentrations: Control (

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: Borosilicate glass, Sterile 250-mL Erlenmeyer flasks covered by inverted glass beakers; Fill volume of 100 ml
- Initial cells density: 1.0 x 10E4 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- No. of vessels per vehicle control (replicates): 3

GROWTH MEDIUM
- Standard medium used: yes

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline


OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 24 hour light
- Light intensity and quality: 400 footcandles (± 10%) or 4300 lux (± 10%)


EFFECT PARAMETERS MEASURED:
Cell Density Measurement Technique: Model Z1 Beckman Coulter® particle counter
Measurement Interval: Daily
Cellular Observations: Visual inspection via light microscope
Observation Interval: Daily

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

0.118 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

act. ingr.

Basis for effect:

growth rate

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

0.024 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

act. ingr.

Basis for effect:

growth rate

Observations:

No physical abnormalities were observed in the controls or treatment groups during the study.

Conclusions:

The 72 and 96-hour growth rates were calculated based on mean measured concentrations. The 72 hour EC50 value for growth rate (ErC50) is 118 μg a.i./L with LOEC and NOEC values of 51.1 and 24.2 μg a.i./L, respectively. The 96-hour EC50 value for growth rate is 134 μg a.i./L with LOEC and NOEC values of 102 and 51.1 μg a.i./L, respectively.

Growth rate:

72 Hour Analysis:
Test Concentration Used for Analysis:	Mean Measured Concentrations
Control Group Comparison:	Pooled
ErC50 (95% Confidence Intervals):	118 μg a.i./L (114 to 123 μg a.i./L)
Treatment Groups Significantly Different (p≤0.5) from Controls:	51.1, 102, 196 and 403 μg a.i./L
Lowest Observed Effect Concentration (LOEC):	51.1 μg a.i./L
No Observed Effect Concentration (NOEC):	24.2 μg a.i./L
Percent Inhibition Range From Controls	24.2 (1.3%), 51.1 (7.8%), 102 (33.8%), 196 (90.9%) and 403 μg a.i./L
	(95.9%)

96 Hour Analysis:
Test Concentration Used for Analysis:	Mean Measured Concentrations
Control Group Comparison:	Pooled
ErC50 (95% Confidence Intervals):	134 μg a.i./L (127 to 142 μg a.i./L)
Treatment Groups Significantly Different (p≤0.5) from Controls:	102, 196 and 403 μg a.i./L
Lowest Observed Effect Concentration (LOEC):	102 μg a.i./L
No Observed Effect Concentration (NOEC):	51.1 μg a.i./L
Percent Inhibition Compared To	24.2 (0.0%), 51.1 (1.7%), 102 (18.9%), 196 (87.8%) and 403 μg a.i./L
Controls:	(94.6%)

Reference 4

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.5400 (Algal Toxicity, Tiers I and II) (January 2012)

Version / remarks:

(2006 draft)

Qualifier:

according to guideline

Guideline:

other: FIFRA Guideline 123-2 (1982)

Principles of method if other than guideline:

The afore mentioned guidelines were harmonized for various test parameters (i.e. temperature, light, etc.) to achieve optimal environmental conditions for the test organism. Scientific discretion was implemented where guideline parameters do not fully converge.

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Vehicle:

yes

Details on test solutions:

Solvent Carrier: Acetone
Solvent Load: 0.1 ml/L

Test organisms (species):

Skeletonema costatum

Test type:

static

Water media type:

saltwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

19.2 to 21.2 °C

pH:

7.7 to 8.5

Salinity:

27 to 27 ppt (all measurements the same)

Nominal and measured concentrations:

Nominal Test Concentrations: Control, Solvent Control, 2.0, 5.1, 13, 32, 80 and 200 μg a.i./L
Mean Measured Test Concentrations: Control (

Details on test conditions:

TEST SYSTEM
- Test vessel:
Test Vessel Size: Sterile 250-mL Erlenmeyer flasks covered by inverted glass beakers
Test Vessel Material: Borosilicate glass
Test Vessel Fill Volume: 100 ml
- Initial cells density: 1.0 x 10E4 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- No. of vessels per vehicle control (replicates): 3

GROWTH MEDIUM
- Standard medium used: yes

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Enriched saltwater (ES) media, according to guideline


OTHER TEST CONDITIONS
- Photoperiod: 16 hours light, 8 hours dark
- Light intensity and quality: 4010 to 4770 lux (measured range) [Cool white fluorescents]
- Salinity (for marine algae): 27 ppt

EFFECT PARAMETERS MEASURED
Parameters Measured (based on cell density): 72 hour: cumulative biomass and growth rate (NOEC, LOEC and EC50)
96 hour: standing crop, cumulative biomass and growth rate (NOEC, LOEC and EC50)
Cell Density Measurement Technique: Manual counts via light microscope and hemocytometer slide
Measurement Interval: Daily
Cellular Observations: Visual inspection via light microscope
Observation Interval: Daily

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

0.064 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

act. ingr.

Basis for effect:

growth rate

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

0.005 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

act. ingr.

Basis for effect:

growth rate

Growth rate:

72 Hour Analysis:
Test Concentration Used for Analysis:	Mean Measured Concentrations
Control Group Comparison:	Pooled
ErC50 (95% Confidence Intervals):	64 μg a.i./L (60 to 68 μg a.i./L)
Treatment Groups Significantly Different (p≤ 0.5) from Controls:	13, 33, 79 and 231 μg a.i./L
Lowest Observed Effect Concentration (LOEC):	13 μg a.i./L
No Observed Effect Concentration (NOEC):	5.1 μg a.i./L
Percent Inhibition Range From Controls	2.0 (-0.4%), 5.1 (-0.3%), 13 (11.6%), 33 (22.1%), 79 (59.5%) and 231  (89.5%) μg a.i./L
96 Hour Analysis:
Test Concentration Used for Analysis:	Mean Measured Concentrations
Control Group Comparison:	Pooled
ErC50 (95% Confidence Intervals):	82 μg a.i./L (76 to 88 μg a.i./L)
Treatment Groups Significantly Different (p≤ 0.5) from Controls:	33, 79 and 231 μg a.i./L
Lowest Observed Effect Concentration (LOEC):	33 μg a.i./L
No Observed Effect Concentration (NOEC):	13 μg a.i./L
Percent Inhibition Compared To   Controls:	2.0 (0.4%), 5.1 (0.1%), 13 (3.1%), 33 (12.3%), 79 (48.5%) and 231   (87.9%) μg a.i./L

Cumulative Biomass (Area Under the Growth Curve):

72 Hour Analysis:
Test Concentration Used for Analysis:	Mean Measured Concentrations
Control Group Comparison:	Pooled
EbC50 (95% Confidence Intervals):	25 μg a.i./L (21 to 29 μg a.i./L)
Treatment Groups Significantly Different (p≤ 0.5) from Controls:	13, 33, 79 and 231 μg a.i./L
Lowest Observed Effect Concentration (LOEC):	13 μg a.i./L
No Observed Effect Concentration (NOEC):	5.1 μg a.i./L
Percent Inhibition Range From Controls	2.0 (1.6%), 5.1 (1.0%), 13 (32.2%), 33 (57.9%), 79 (90.7%) and 231 (98.4%) μg a.i./L
96 Hour Analysis:
Test Concentration Used for Analysis:	Mean Measured Concentrations
Control Group Comparison:	Pooled
EbC50 (95% Confidence Intervals):	29 μg a.i./L (25 to 33 μg a.i./L)
Treatment Groups Significantly Different (p≤ 0.5) from Controls:	13, 33, 79 and 231 μg a.i./L
Lowest Observed Effect Concentration (LOEC):	13 μg a.i./L
No Observed Effect Concentration (NOEC):	5.1 μg a.i./L
Percent Inhibition Range From Controls	2.0 (1.8%), 5.1 (1.1%), 13 (26.3%), 33 (53.2%), 79 (91.3%) and 231 (98.9%) μg a.i./L

Observations:

No physical abnormalities were observed in the controls or treatment groups during the study.

Conclusions:

The 72 and 96-hour growth rates were calculated based on mean measured concentrations. The 72 hour EC50 value for growth rate (ErC50) is 64 μg a.i./L with LOEC and NOEC values of 13 and 5.1 μg a.i./L, respectively. The 96-hour EC50 value for growth rate is 82 μg a.i./L with LOEC and NOEC values of 33 and 13 μg a.i./L, respectively.

Reference 5

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Version / remarks:

(2006)

Deviations:

no

Remarks:

, No significant deviations in the study

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.5400 (Algal Toxicity, Tiers I and II) (January 2012)

Version / remarks:

(2006 draft)

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: FIFRA Guideline 123-2 (1982)

Deviations:

no

Principles of method if other than guideline:

The afore mentioned guidelines were harmonized for various test parameters (i.e. temperature, light, etc.) to achieve optimal environmental conditions for the test organism. Scientific discretion was implemented where guideline parameters do not fully converge.

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
The TGAS coded AE 1170437 used at the initiation of this study includes multiple isomers, including the isomers AE 1170437 (principle active substance) and AE 1170438 (active isomer of the principle active substance). Since the biological activity of AE 1170438 is similar to AE 1170437, both are regarded as active substances in the TGAS. The TGAS contains 95% (94.7%) to 100% AE 1170437 and, 0 % to 5 % (5.3 %) AE 1170438. In order to distinguish between the single diastereomer AE 1170437, and the TGAS containing both AE 1170437 and AE 1170438, a new BCS code, BCS-AA10717, has been designated for the TGAS mixture of stereoisomers. All references to the technical active substance and analytical results as AE 1170437 are equivalent to the newly established TGAS: BCS-AA10717.

Analytical monitoring:

yes

Vehicle:

yes

Details on test solutions:

see meterials and methods

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM
- Common name: green algae
- Source: Own culture originally optained from University of Texas (UTEX), Austin, Texas, USA, received
February 13, 2007

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

23.9 to 24.2 °C

pH:

7.3 to 10.0 (Initial pH of media: 7.5 ± 0.1)

Nominal and measured concentrations:

Nominal Test Concentrations: Control, Solvent Control, 25, 50, 100, 200 and 400 μg a.i./L
Initial Measured Test Concentrations: Control (

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: Borosilicate glass; Sterile 250-mL Erlenmeyer flasks covered by inverted glass beakers, fill volume of 100 mg/L
- Initial cells density: 1.0 x 10E4 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- No. of vessels per vehicle control (replicates): 3

GROWTH MEDIUM
- Standard medium used: yes

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 24 hour light
- Light intensity and quality: 4480 – 4900 lux (Cool white fluorescents)


EFFECT PARAMETERS MEASURED:
- Determination of cell concentrations:
Cell Density Measurement Technique: Manual counts via light microscope and hemocytometer slide
Measurement Interval: Daily
Cellular Observations: Visual inspection via light microscope
Observation Interval: Daily

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

0.112 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

act. ingr.

Basis for effect:

growth rate

Remarks on result:

other: (95% CI: 98 to 125 μg a.i./L)

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

0.02 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

act. ingr.

Basis for effect:

growth rate

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

0.117 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

act. ingr.

Basis for effect:

growth rate

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

0.039 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

act. ingr.

Basis for effect:

growth rate

The 72 and 96-hour growth rates were calculated based on initial measured concentrations. The 72 hour EC50 value for growth rate (ErC50) is 112 μg a.i./L with LOEC and NOEC values of 39

and 20 μg a.i./L, respectively. The 96-hour EC50 value for growth rate is 117 μg a.i./L with LOEC and NOEC values of 79 and 39 μg a.i./L, respectively.

---

Observations: No physical abnormalities were observed in the controls or treatment groups during the study

Growth rate:

0 to 72 Hour Analysis
Test Concentration Used for Analysis:	Initial measured concentrations
Control Group Comparison:	Pooled
ErC50 (95% Confidence Intervals):	112 μg a.i./L (98 to 125 μg a.i./L)
Treatment Groups Significantly Different (p≤0.5) from Controls:	39, 79, 159 and 242 μg a.i./L
Lowest Observed Effect Concentration (LOEC):	39 μg a.i./L
No Observed Effect Concentration (NOEC):	20 μg a.i./L
Percent Inhibition Range From Controls (Table5):	20 (0.7%), 39 (3.1%), 79 (17.4%), 159 (82.0%) and 242 (84.5%) μg a.i./L
0 to 96 Hour Analysis:
Test Concentration Used for Analysis:	Initial measured concentrations
Control Group Comparison:	Pooled
ErC50 (95% Confidence Intervals):	117 μg a.i./L (104 to 129 μg a.i./L)
Treatment Groups Significantly Different (p≤0.5) from Controls:	79, 159 and 242 μg a.i./L
Lowest Observed Effect Concentration (LOEC):	79 μg a.i./L
No Observed Effect Concentration (NOEC):	39 μg a.i./L
Percent Inhibition Compared To Controls:	20 (1.5%), 39 (0.1%), 79 (9.8%), 159 (81.4%) and 242 (88.1%) μg a.i./L

Description of key information

The substance is toxic to algae.

Key value for chemical safety assessment

EC50 for freshwater algae:

0.112 mg/L

EC50 for marine water algae:

0.064 mg/L

EC10 or NOEC for freshwater algae:

0.02 mg/L

EC10 or NOEC for marine water algae:

0.005 mg/L

Additional information

Five studies with the test substance are available for the toxicity to algae, all studies referred to herein were conducted in accordance with the prevailing OECD testing guidelines (OECD 201) and GLP. Based on measured concentrations, the 96h EC50 (growth rate) in the green algae Pseudokirchneriella subcapitata was calculated to be 0.112 mg/L test item. The NOEC (growth rate) was at 0.02 mg/L. Another study with Pseudokirchneriella subcapitata exhibited an EC50 (72h) of 0.118 mg/L and a NOEC of 0.024. The 96h EC50 in the saltwater diatom Skeletonema costatum for the growth rate is 0.064 mg/L and for Navicula pelliculosa was an EC50 of 0.240 mg/L revealed the 96 h EC50 for the blue green algae Anabaena flos-aquae was 3 mg/L.Therefore the substance proved to be toxic to green algae, blue green algae and saltwater algae species as well as freshwater diatom algae as evidenced by EC50 values for the growth rate ranging from 0.06 to 3 mg/L.