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EC number: 428-650-4 | CAS number: 153719-23-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic plants other than algae
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic plants other than algae
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Test organisms (species):
- Lemna gibba
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 90.2 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- frond number
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The 7-day EC50 for growth rate (ErC50) for the test substance to Lemna gibba was >90.2 mg test substance/L, based on mean measured concentrations.
- Executive summary:
The toxicity of the test substance to the aquatic plant Lemna gibba was determined in a 7-day semi-static test according to the GLP compliantASTM Guideline E 1415 - 91study. The Lemn awere exposed to nominal concentrations of 3.13, 6.25, 12.5, 25, 50 and 100 mg test substance/L, equivalent to 2.90, 5.70, 11.4, 22.0, 43.9 and 90.2 mg test substance/L as mean measured concentrations. Assessments of frond number were made on days 0, 3, 5 and 7. Fronds were harvested for measurement of dry weight after 7 days. Temperature was measured continuously, light intensity was recorded at the start and end of the test and pH was recorded on days 0, 3 and 7. The test concentrations were verified by chemical analysis of the test substance on days 0, 3 (old and new solutions) and 7, using high performance liquid chromatography with ultra violet detection at 250 nm. For frond number, the 7-day EC50 for growth rate (ErC50) for the test substance to Lemna gibba was >90.2 mg test substance/L, based on mean measured concentrations. For dry weight, the 7-day EC50 for growth rate (ErC50) was not stated. This study complies with the current validity criteria for toxicity testing with aquatic plants, despite more than the recommended number of fronds being added per vessel at test start.
Reference
At the start of the test, the concentrations of the test item were found to be in the range 97.6 to 99.2 % of the nominal values and at the end of the test were in the range 70.0 to 77.76%. Mean measured concentrations were used for the calculation and reporting of results.
Analytical results
Mean measured concentrations |
% of nominal measured on day 0 |
% of nominal (old solutions) measured on day 3 |
% of nominal (new solutions) measured on day 3 |
% of nominal measured on day 7 |
0 (control) |
<LOD |
<LOD |
<LOD |
<LOD |
2.9 |
99.0 |
89.5 |
102.2 |
73.5 |
5.7 |
97.6 |
94.4 |
96.0 |
73.6 |
11.4 |
99.2 |
86.4 |
100.0 |
77.6 |
22.0 |
98.4 |
84.8 |
98.8 |
70.4 |
43.9 |
98.6 |
85.0 |
97.8 |
70.0 |
90.2 |
98.9 |
85.1 |
99.3 |
77.5 |
Data for frond number and dry weight were used to calculate growth rates for the control and each exposure concentration. Logit analysis was then used to calculate the 7-day ErC50, based on percent inhibition relative to the control. For the No Observed Effect Concentration and Lowest Observed Effect Concentration, a Dunnett’s Test was used to determine values significantly different to the control.
Mean frond numbers are presented below along with the growth rate, yield and respective inhibition values, alongside estimated EC50values:
Effect of the test item on growth rate and yield (frond number) of Lemna gibba
Mean measured concentrations |
Mean No. fronds/replicate (day 7) |
Based on Frond Number (0-7 days) |
|||
Growth Rate |
Inhibition of Growth Rate (%) |
Yield |
Inhibition of Yield (%) |
||
0 (control) |
181 |
0.00 |
Not reported |
Not reported |
Not reported |
2.9 |
169 |
4.51 |
Not reported |
Not reported |
Not reported |
5.7 |
184 |
0.04 |
Not reported |
Not reported |
Not reported |
11.4 |
172 |
4.14 |
Not reported |
Not reported |
Not reported |
22.0 |
177 |
2.45 |
Not reported |
Not reported |
Not reported |
43.9 |
185 |
0.91 |
Not reported |
Not reported |
Not reported |
90.2 |
177 |
2.65 |
Not reported |
Not reported |
Not reported |
EC50mg/L |
>90.2 (mean measured) |
n.d. |
|||
95% confidence limits |
n.d. |
n.d. |
|||
NOEC (mg/L) |
90.2 |
n.d. |
|||
LOEC |
n.d. |
n.d. |
Inoculum
= 18 - 21 fronds
n.d.
– Not determined
Mean
dry weights are presented below along with the growth rate, yield and
respective inhibition values, alongside estimated EC50 values:
Effect of the test item on growth rate and yield (dry weight) of Lemna gibba
Mean measured concentrations |
Dry Weight (mg) (day 7) |
Based on Dry Weight (0-7 days) |
|||
Growth Rate |
Inhibition of Growth Rate (%) |
Yield |
Inhibition of Yield (%) |
||
0 (control) |
29.6 |
Not reported |
Not reported |
Not reported |
Not reported |
2.9 |
25.1 |
Not reported |
Not reported |
Not reported |
Not reported |
5.7 |
29.3 |
Not reported |
Not reported |
Not reported |
Not reported |
11.4 |
25.9 |
Not reported |
Not reported |
Not reported |
Not reported |
22.0 |
26.0 |
Not reported |
Not reported |
Not reported |
Not reported |
43.9 |
31.5 |
Not reported |
Not reported |
Not reported |
Not reported |
90.2 |
27.1 |
Not reported |
Not reported |
Not reported |
Not reported |
EC50mg/L |
n.d. |
n.d. |
|||
95% confidence limits |
n.d. |
n.d. |
|||
NOEC |
90.2 |
n.d. |
|||
LOEC |
n.d. |
n.d. |
Inoculum = Not reported
n.d. – Not determined
For frond number, the 7-day EC50 for growth rate (ErC50) for the test substance to Lemna gibba was >90.2 mg test substance/L, based on mean measured concentrations. For dry weight, the 7-day EC50 for growth rate (ErC50) was not stated. This study complies with the current validity criteria for toxicity testing with aquatic plants, despite more than the recommended number of fronds being added per vessel at test start. There is no impact on the reliability of the study and therefore it is still valid for use in the risk assessment
Description of key information
7-day EC50 for growth rate (ErC50) for the test substance to Lemna gibba was >90.2 mg test substance/L, ASTM E 1415 -91, Grade 1998
Key value for chemical safety assessment
Additional information
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