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EC number: 227-876-9
CAS number: 6021-61-0
Considering that test substance could
influence the photosynthesis capacity of algae cells in the test
solution, it was considered a test using Lemna as a test organism.
Coloured substances are often
assumed to behave differently in the algal growth inhibition test due to
shading of the algae. Coloured substances can absorb photosynthetically
active light and hence limit growth of algal cultures. Absorption will
be proportional to test substance concentration and as a consequence it
can result in growth inhibition which is difficult to distinguish from
Several studies on algae conducted
on dark dyes, including those with a modified test system for
dye-stuffs, showed that the growth inhibition is mainly correlated to
the reduced light absorption, caused by the stained water and not due to
an effective toxicity of the dye.
A modified test design has been
proposed by the cooperation of the Ecological and Toxicological
Association of Dyes and Organic Pigments Manufacturers (ETAD) (Memmert
et al) in order to eliminate the effect of shading. Nevertheless this
method has some limitations, as it focuses on the shadow effect, giving
no information on the real potential toxicity for algae of the tested
Considering that the test substance
is a dyestuff, which could influence the photosynthesis capacity of
algae cells in the test solution, and in order to clarify the
(eco)toxicological properties related to toxicity for aquatic plants, it
was considered a test using Lemna as a test organism, according to the
OECD guideline 221, as suggested also in the Guidance on Information
Requirements and Chemical Safety Assessment Chapter R.7b: Endpoint
specific guidance, published by the European Chemical Agency.
Memmert U, Motschi H, Inauen J, W.
Uthrich V. Inhibition of algal growth caused by coloured test
substances. ETAD Project E 3023. Ecological and Toxicological
Association of Dyes and Organic Pigments Manufacturers, Basel,
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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