2-[4-[(2-chloro-4-nitrophenyl)azo]-N-(2-cyanoethyl)anilino]ethyl acetate

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

February from 09th to 21st, 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Version / remarks:

adopted July 27, 1995

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

column elution method

Water solubility:

94 µg/L

Conc. based on:

test mat.

Temp.:

20 °C

pH:

7.2

Details on results:

The water solubility of test item was determined to be 0.094 ± 0.00924 mg/l (95 % confidence interval) at 20 ± 0.5 °C using the column elution method for the performance of the main test.

The measured concentrations are constant within ± 30 % and thus within the range required by the guidelines.

Results of the determination of water solubility of test item (flow rate: ca. 25 ml/hour)

Measured concentration (mg/l)
Column 1		Column 2
below the quantitation limit	pH = 7.44	below the quantitation limit	pH = 7.31
below the quantitation limit	pH = 7.42	below the quantitation limit	pH = 7.28
below the quantitation limit	pH = 7.45	below the quantitation limit	pH = 7.29
0.0622	pH = 7.40	0.0665	pH = 7.27
0.0701	pH = 7.38	0.0695	pH = 7.31
0.0961	pH = 7.23	0.0989	pH = 7.20
0.0977	pH = 7.19	0.1139	pH = 7.21
0.0939	pH = 7.15	0.0978	pH = 7.18
0.0888	pH = 7.07	0.0958	pH = 7.15
Mean / RDS: 0.0876 / 19 %
pH 7.22 - 7 -24

Results of the determination of water solubility of test item (flow rate: ca. 12.5 ml/hour)

Measured concentration (mg/l)
Column 1.		Column 2.
0.0978	pH = 7.19	0.1070	pH = 7.23
0.1088	pH = 7.20	0.1080	pH = 7.18
0.1221	pH = 7.20	0.1215	pH = 7.21
0.0916	pH = 7.17	0.1055	pH = 7.18
0.1072	pH = 7.13	0.0891	pH = 7.15
0.0961	pH = 7.14	0.1118	pH = 7.16
0.0759	pH = 7.10	0.0699	pH = 7.09
0.0830	pH = 7.10	0.0869	pH = 7.09
Mean / RDS: 0.0989 / 15 %
pH 7.15 - 7 .16

Conclusions:

Water solubility: 94 µg/l at 20 °C and pH ca 7.20

Executive summary:

The purpose of the study was to evaluate the solubility of test item in water at 20 ± 0.5 °C, according to the OECD guideline 105. The method chosen is based on the elution of the test substance with water from a micro-column, which is charged with an inert support material coated with an excess of test substance. The water solubility is determined when the mass concentration of the eluate is constant, i.e. concentration plateau as a function of time.

The water solubility of test item was determined to be 0.094 ± 0.00924 mg/l (95 % confidence interval) at 20 ± 0.5 °C using the column elution method for the performance of the main test. The measured concentrations are constant within ± 30 % and thus within the range required by the guidelines.

Conclusion

Water solubility: 94 µg/l at 20 °C and pH ca 7.20.

Description of key information

94 µg/l at 20 °C and pH ca. 7.20

Key value for chemical safety assessment

Water solubility:

94 µg/L

at the temperature of:

20 °C

Additional information

OECD 105 - column elution method