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Toxicity to microorganisms

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Reference
Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1996
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
study conducted under GLP and according to DIN 38412 part 27 (1992). According to ECHA endpoint specific guidance chapter 7b (2012), subchapter toxicty tests with bacteria (page 149), "In general, preference is given to tests with a mixed inoculum that assess the functioning of the entire microbial community in an STP, rather than tests based on single species or even microbial sub-systems ... Often inhibition test data on individual bacterial species may be available. Results of the cell multiplication inhibition test with P. putida (Bringmann and Kühn 1980) should be used for calculation of the PNECmicro-organisms only in cases where no other test results are available."
Qualifier:
according to
Guideline:
other: DIN-38412, part 27 (1992)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
no
Details on sampling:
- Concentrations:
Preparation of test water by direct weighing of the test substance into the test vessels and ultrasonic treatment before the bacteria suspension was added
- Sampling method: not reported
- Sample storage conditions before analysis: not applicable
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: direct weighing and mixing into test vessel and ultrasonic treatment
- Eluate: not applicable
- Differential loading: not applicable
- Controls: yes (blind values)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): no vehicle used
- Evidence of undissolved material (e.g. precipitate, surface film, etc): up to and including 10 mg/L clear solution, turbid suspension at higher test concentration with an observable separation of phases
Test organisms (species):
Pseudomonas putida
Details on inoculum:
- Laboratory culture: TBB-Microbiology, Henkel KGaA
- Method of cultivation: not reported
- Preparation of inoculum for exposure: 1-3 mL bacterial suspension added to the test solution composed of test water (according to DIN 38412, part 27. point 8.1), 2 mL Glucose solution and test substance
- Pretreatment: bacterial suspension 2-3 days old
- Initial biomass concentration: not reported
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3.8 min
Remarks on exposure duration:
observation times different in the treatments dependent on the decrease rate of oxygen
Hardness:
not reported
Test temperature:
21.2 °C
pH:
not reported
Dissolved oxygen:
endpoint of the study: change of oxygen concentration
Salinity:
not applicable
Nominal and measured concentrations:
main test 1: nominal 300 and 10000 mg/L
main test 2: nominal 10, 30 and 100 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Erlenmeyer 100 mL
- Type: not reported
- Material, size, headspace, fill volume: glass. 100 mL, 100 mL
- Aeration: direct
- No. of organisms per vessel: 1-3 mL bacteria suspension
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dilution water according to DIN 38412, part 27, point 8.1
- Total organic carbon: not reported
- Particulate matter: not reported
- Metals: not reported
- Pesticides: not reported
- Chlorine: not reported
- Alkalinity: not reported
- Ca/mg ratio: not reported
- Conductivity: not reported
- Culture medium different from test medium: not reported
- Intervals of water quality measurement: not applicable

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: not reported
- Light intensity: not reported

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): change in oxygen concentration

TEST CONCENTRATIONS
- Spacing factor for test concentrations:
main test 1: 33.3
main test 2: 3-3.3
Reference substance (positive control):
no
Key result
Duration:
3.8 min
Dose descriptor:
EC0
Effect conc.:
30 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: decrease in oxygen consumption
Key result
Duration:
3.8 min
Dose descriptor:
EC10
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: decrease of oxygen consumption

Table 1 Oxygen consumption in main test 1

Blind value  test concentration (mg/L)  replicates  measurement (mg O2 per L and per min
 1  ---  ---  2.09
 2  ---  ---  2.07
 ---  10000  1  1.58
 ---  10000  2  1.66
 ---  300  1  1.51
 ---  300  2  1.48
 3  ---  ---  1.75
 4  ---  ---

 1.87

Table 2 Oxygen consumption in main test 2

 Blind value  test concentration (mg/L)  replicates  measurement (mg O2 per L and per min
5  ---  --- 1.93
 6  ---  --- 2.21
 --- 30  1  1.82
 --- 30  2  1.99
 --- 10  1  1.98
 --- 10  2  2.21
 --- 100  1  1.80
--- 100 2

 1.92

 7  ---  ---  1.96
 8  ---  ---  2.15

Table 3 Oxygen consumption relative to the blind values

 Test concentration (mg/L)  Mean of inhibition (+) or advancement (-) from two replicates (%)
 10 -1.5 
 30  +7.6
 100  +9.8
 300  +23.1
 10000  +16.8
Validity criteria fulfilled:
yes
Conclusions:
In a valid and reliable study according to DIN 38412, part 27 (1992), the inhibition of oxygen consumption of Pseudomonas putida was measured. The EC0 and EC10 were 30 and 100 mg/L, respectively.
Executive summary:

The acute toxicity of the test substance Sandelice to bacteria was determined using the test for inhibition of the oxygen consumption according to DIN 38412, part 27 (1992). The tested concentration range was 10 -10000 mg/L. The following effect concentrations were determined:

EC0: 30 mg/L

EC10: 100 mg/L

Description of key information

The acute toxicity of the test substance to bacteria was determined using the test for inhibition of the oxygen consumption according to DIN 38412, part 27 (1992). The tested concentration range was 10 -10000 mg/L.

The following effect concentrations were determined:

EC0: 30 mg/L

EC10: 100 mg/L

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:
100 mg/L

Additional information