2-ethyl-4-methylimidazole

Specific investigations: other studies

Administrative data

Endpoint:

biochemical or cellular interactions

Type of information:

experimental study

Adequacy of study:

disregarded due to major methodological deficiencies

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Test design not relevant for any toxicological endpoint. Basic data given.

Data source

Reference

Reference Type:

publication

Title:

Reduction of Induced Mutability with Xanthine- and Imidazole-derivatives through Inhibition of Metabolic Activation

Author:

Yamaguchi T. and Nakagawa K.

Year:

1983

Bibliographic source:

Agric. Biol. Chem., 47 (7), 1673-1677

Materials and methods

Principles of method if other than guideline:

Inhibitory test on mutagenicity: A modified Ames mutation assay was performed using Salmonella typhimurium TA 98 and TA 100 as test strains. Cells were treated with a known mutagen and a sample solution and the mutagenicity was tested by the standard method. The result was compared with a control in which distilled water was used instead of the sample solution.

GLP compliance:

no

Type of method:

in vitro

Test material

Test material information

Constituent 1

Reference substance name:

2-ethyl-4-methylimidazole

EC Number:

213-234-5

EC Name:

2-ethyl-4-methylimidazole

Cas Number:

931-36-2

Molecular formula:

C6H10N2

IUPAC Name:

2-ethyl-4-methyl-1H-imidazole

Details on test material:

Name of test substance (as cited in study report): 2-Ethyl-4-methylimidazol

Test animals

Species:

other: Salmonella typhimurium

Strain:

other: TA 98, TA 100

Administration / exposure

Details on study design:

Cells of Salmonella typhimurium TA 98 or TA 100 were treated at 37°C for 20 min with 0.1 mL of mutagen, with or without 0.25 mL of S-9 mix and 0.1 mL of a sample solution and the mutagenicity was tested by the standard method. The result was compared with a control in which distilled water was used instead of the sample solution.

Results and discussion

Details on results:

In contrast to imidazole and 1-methylimidazole, 2-Ethyl-4-methylimidazole did not lead to a reduction in TRP-P-2, acetylaminofluorene and benzo(a)pyrene induced mutagenicity in Salmonella typhimurium TA100 and TA98.