Registration Dossier
Registration Dossier
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EC number: 604-351-6 | CAS number: 143390-89-0
Toxicity to terrestrial arthropods
Administrative data
- Endpoint:
- toxicity to terrestrial arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 213 (Honeybees, Acute Oral Toxicity Test)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 214 (Honeybees, Acute Contact Toxicity Test)
- GLP compliance:
- yes
- Application method:
- other: contact and oral
Test material
- Reference substance name:
- methyl (2E)-2-methoxyimino-2-[2-[(2-methylphenoxy)methyl]phenyl]acetate
- EC Number:
- 604-351-6
- Cas Number:
- 143390-89-0
- Molecular formula:
- C18 H19 N O4
- IUPAC Name:
- methyl (2E)-2-methoxyimino-2-[2-[(2-methylphenoxy)methyl]phenyl]acetate
- Details on test material:
- - Name of test material (as cited in study report): BAS 490 F
- Physical state: light brown solid
- Analytical purity: 97.8% ± 1.0% active ingredient
- Batch No.: COD-000225
- Storage condition of test material: at room temperature
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test substrate
- Vehicle:
- yes
Test organisms
- Test organisms (species):
- Apis mellifera
- Animal group:
- Hymenoptera (honeybees)
- Details on test organisms:
- TEST ORGANISM
- Common name: worker honey bees
- Source: Honey bee colonies, disease-free and queen-right, bred by IBACON GmbH, Darmstadt, Germany
- Age at test initiation: adult
- Sex: female
- Cultural background:
- Disease free: yes
- Kept according to standard practices: yes
Study design
- Study type:
- laboratory study
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- 25 - 26 °C
- Humidity:
- 37-70%
- Photoperiod and lighting:
- Darkness (expect during observation)
- Details on test conditions:
- TEST SYSTEM
Type: stainless steel cages
Size: 10 x 8.5 x 5.5. cm (length x width x height)
No. of individuals/unit: 10
Replicates: 5 per test item dose, controls and reference item dose
Food: Commercial ready-to-use syrup (Apiinvert; 30 % Saccharose, 31 % Glucose, 39 % Fructose) ad libitum; was given directly after treatment using syringes; no replacements of the food was necessary during the experimental time of the experiments (48 h)
TEST PARAMETERS: mortality, behavioural abnormalities after 4, 24, 48 hours - Nominal and measured concentrations:
- Nominal dose level of the test item in the contact test and the oral test: 100 µg a.i./bee
Measured dose level of the test item in the oral test: 111.0 µg a.i./bee
Nominal dose level of the reference item in the contact test: 0.3, 0.2, 0.15, 0.1 µg dimethoate per bee
Nominal dose level of the reference item in the oral test: 0.3, 0.15, 0.08, 0.05 µg dimethoate per bee
Measured dose level of the reference item in the oral test: 0.28, 0.15, 0.08, 0.05 µg dimethoate per bee - Reference substance (positive control):
- yes
- Remarks:
- dimethoate 400 mg/L (nominal)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- LD50
- Effect conc.:
- > 100 µg per animal
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Remarks on result:
- other: contact test
- Duration:
- 48 h
- Dose descriptor:
- LD50
- Effect conc.:
- > 111 µg per animal
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Remarks on result:
- other: oral test
- Details on results:
- CONTACT TOCICITY
- Mortality in the water control: none
- Mortality in the solvent control: none
- Behavioural abnormalities: none
ORAL TOCICITY
- Mortality in the water control: none
- Mortality in the solvent control: none
- Behavioural abnormalities: none - Results with reference substance (positive control):
- Contact LD50 (48 h) = 0.19 µg/bee
Oral LD50 (48 h) = 0.15 µg/bee
Applicant's summary and conclusion
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