4-tert-butylbenzaldehyde

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP-guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

4-tert-butylbenzaldehyde

EC Number:

213-367-9

EC Name:

4-tert-butylbenzaldehyde

Cas Number:

939-97-9

Molecular formula:

C11H14O

IUPAC Name:

4-tert-butylbenzaldehyde

Details on test material:

- Name of test material (as cited in study report): 4-tert-butylbenzaldehyde
- Lot/batch No.: Inprozessprobe aus Behaelter B1 vom 19.09.2007
- Analytical purity: 96.4 area %
- Physical state: liquid

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:10.08 mg and 10.1 mg test substance were added to 2 liters of test medium. The solution was stirred for 20 minutes at 20+/- 2 °C on a magnetic stirr plate. The test solutions were prepared by dilution the stock solution.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age at study initiation (mean and range, SD): 2 - 24 h (Age of stock animals: 2 - 4 weeks)

Study design

Test type:

other: Static-renewal

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.70 +/- 0.50 mmol/l

Test temperature:

20 +/- 1 °C

pH:

8.0 - 8.2

Dissolved oxygen:

8.7 - 9.1 mg/l

Nominal and measured concentrations:

Nominal test concentrations (mg/l):
0.1, 0.22, 0.5, 1.0, 2.2, 5.0, control

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beakers, nominal volume: 100 ml
- Fill volume: 60 ml
- Renewal rate of test solution (frequency/flow rate): the test solution was changed after 24 h
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.08 animals/ml

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: A synthetic fresh water was used for culture and test purposes (Elendt M4 according to OECD 202).
- Conductivity: 594 µS/cm


OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day night regime


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
mobility after 0, 24 and 48 h

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

0.22 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.494 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

0.113 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 5

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.277 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 6

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

0.5 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

EC50 (24 h): 0.63 mg/l

Reported statistics and error estimates:

The EC50 (48 h) was calculated using the Probit analyses (Finney, 1971)

Any other information on results incl. tables

The number of mobile daphnids after hours of exposure in the different  test concentrations was as following:

Concentration   Number of immobile Daphnia after x hours
[mg/l]           0        24          48
  0              0         0           0
  0.1            0         0           0   
  0.22           0         0           0   
  0.5            0         1          11
  1.0            0         1          20
  2.2            0         5          20
  5.0            0        20          20