6,6',6''-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoic acid, compound with 2,2',2''-nitrilotriethanol (1:3)

Toxicity to terrestrial arthropods

Link to relevant study record(s)

Reference

Endpoint:

toxicity to terrestrial arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study meets generally accepted scientific principles, comparable to guideline study, acceptable for assessment

Qualifier:

equivalent or similar to guideline

Guideline:

other: OECD TG 477 Genetic Toxicology: Sex-Linked Recessive Lethal Test in Drosophila melanogaster

Principles of method if other than guideline:

Method: other: Feeding Experiment

GLP compliance:

not specified

Application method:

oral

Analytical monitoring:

not specified

Vehicle:

no

Test organisms (species):

Drosophila melanogaster

Animal group:

Diptera (fruit fly (small))

Details on test organisms:

- test animals: Drosophila melanogaster; Canton-S males and Basc females

Study type:

laboratory study

Limit test:

no

Total exposure duration:

3 d

Details on test conditions:

- route of application: feeding for 3 d
TEST SYSTEM
- Test container (material, size): glass shell vials
- Amount of soil or substrate: none
- male flies were treated for 3 days in glass shell vials containing a glass fiber disc soaked with 0.2-0.5 ml of the test solution

- Test concentrations: 20000 ppm (ca. 22200 mg/l), 30000 ppm (ca. 33300 mg/l)

Reference substance (positive control):

not specified

Duration:

3 d

Dose descriptor:

LC50

Effect conc.:

ca. 49 950 mg/kg soil dw

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

mortality

RS-Freetext:
Dose    Mortality   Sterility
(ppm)   (%)         (%)
20000    2           3
30000   48          20

Description of key information

The compound consists of a triazine part and an amine part. Under environmental conditions it is present in its dissociated form. Data on the toxicity to terrestrial arthropods are only available for the amine part CAS 102-71-6.   The test reveals a low hazard potential to terrestrial organisms.

Additional information

The compound consists of a triazine part and an amine part. Under environmental conditions it is present in its dissociated form. Data on the toxicity to terrestrial arthropods are only available for the amine part CAS 102-71-6.

Only one valid study was performed by Yoon et al. (1985). The test was conducted comparable to OECD TG 477. As this test is usually used for testing on genotoxic effects on fruit flies (Drosophila spec.) lethal effects of substances may be tested in parallel. The test organisms were fed with with a stock solution of known concentration. After 3 days of exposure, a mortality of 48% (= LC48) was reported for a concentration of ca. 33300 mg/l.

However, this test can not be used for the derivation of the PNECsoil, since Drosophila adult male animals were treated with the test substance at rather high concentrations. Interaction with soil was not guaranteed and therefore, it may not be concluded, that the exposure path was the one required for the derivation of the PNECsoil.