6,6',6''-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoic acid, compound with 2,2',2''-nitrilotriethanol (1:3)

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2012

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with detailed documentation

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

EL

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna Straus
- Source: own rearing, original source Water Research Institute, Bratislava, Slovak Republic
- Age at study initiation (mean and range, SD): < 24 h
- Feeding during test: no

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test temperature:

21.2 - 22.0 °C

pH:

8.14 - 8.20

Dissolved oxygen:

6.01 - 6.65 mg/L

Nominal and measured concentrations:

3300 mg/L (nominal concentration, initial)
measured concentrations: From the results of UV/Vis spectrometric measurements is evident that the concentration of the substance has been satisfactorily maintained within required +-20% of the nominal concentration throughout the test. (Deviation of nominal : + 6.7%/ +16.1% l at beginning/ end of test)

Details on test conditions:

TEST SYSTEM
- Test vessel: 10 mL
- Type: closed
- Material: glass or other chemically inert material
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 2 mL/animal

- Holding water: dechlorinated tap water (Chlorine: < 10 µg/L, 151 mg/L (CaCO3))

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ISO 6341 test water which was aereated before use.
- Intervals of water quality measurement: oxygen and pH measured at beginning and end of test

OTHER TEST CONDITIONS
- Adjustment of pH: yes, 1 N NaOH to pH 8.2 to ensure solubility of the test substance
- Photoperiod: 16 h light / 8 h dark

EFFECT PARAMETERS MEASURED:
immobilisation recorded at 24 and 48 h

TEST CONCENTRATIONS
- 3300 mg/L
- Range finding study: yes

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 3 300 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

- 48 h-EC50: 0.68 mg/L (mean of 3 determinations)

Immobilisation at 3300 mg/L: 0% after 24 h, 10% after 48 h

Validity criteria fulfilled:

yes

Executive summary:

The short term toxicity of TC to Daphnia magna was tested according to OECD guideline 202. 10% immobilisation was observed within 48 h at the only tested concentration of 3300 mg/L. Therefore, the 48h-EC50 was determined to be >3300 mg/L.

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study meets generally accepted scientific principles, well documented, according to national australian guideline wich is comparable to more widespread used guidelines. acceptable for assessment.

Qualifier:

according to guideline

Guideline:

other: New South Wales Gouvernment Environment Protection Authority: ASTM Designation E1192 (1988)

GLP compliance:

not specified

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stock solutions, stirred in the dark; diluted to the appropriate concentrations immediately prior to the commencement of the test.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): no

Test organisms (species):

Ceriodaphnia dubia

Details on test organisms:

TEST ORGANISM
- Common name: Ceriodaphnia cf. dubia
- Source: laboratory
- Age at study initiation (mean and range, SD): < 24 h
- Method of breeding: cultures were maintained in 2-L glass beakers and transferred to fresh water three times weekly. Food was provided after water renewal (unicellular algae: Pseudokircheneriella subcapitata and Ankistrodesmus sp., each at 25000 cells/mL)
- Feeding during test: no

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

23±1 °C

Nominal and measured concentrations:

series of 5 test concentrations plus control

Details on test conditions:

TEST SYSTEM
- Test vessel: beakers
- Material, size, headspace, fill volume: 250-mL glass beakers, fill volume: 200 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- Biomass loading rate: 1 animal / 40 mL

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tapwater, filtered (1 µm), aged (1 month), adjusted to 500 µS/cm with seawater


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 h light:dark cycle
- Light intensity: 1000 lux at surface

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- measurements: 
- number of immobile cladocera counted at the end of test                  
- pH, dissolved oxygen, conductivity at test initiation before test animals were added and at test completion

TEST CONCENTRATIONS
- Spacing factor for test concentrations: no data
- Justification for using less concentrations than requested by guideline: not applicable
- Range finding study: performed
- Test concentrations: no data

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

609.88 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 95% confidence interval 565.2 mg/l - 658.3 mg/l

Reported statistics and error estimates:

The 48-h EC50 (immobilisation) values and the corresponding 95 % confidence intervals were based on nominal concentrations and were determined by the trimmed Spearman-Kaerber method (Hamilton et al., 1977).

Validity criteria fulfilled:

yes

Description of key information

The compound consists of a triazine part and an amine part. Under environmental conditions it is present in its dissociated form. Therefore, separate assessments were conducted for the triazine and the amine part, respectively.  

CAS 80584-91-4: There is a high probability that the compound is not acutely harmful to aquatic invertebrates.  

CAS 102 -71 -6: There is a high probability that the compound is not acutely harmful to aquatic invertebrates.

Additional information

The compound consists of a triazine part and an amine part. Under environmental conditions it is present in its dissociated form. Therefore, separate assessments were conducted for the triazine and the amine part, respectively.

CAS 80584-91-4

The acute toxicity to invertebrates was assessed in a 48-h acute toxicity to Daphnia magna study in accordance to OECD guideline 202. The test was conducted as limit test under static conditions with a concentrations of 3300 mg/L. The 48-h EC50 was determined to be > 3300 mg/L. Additionally, supporting data on the acute toxicity to invertebrates according to OECD 202 within 24-h is available. The test was conducted under static conditions with measured concentrations of 10, 18, 32, 58, 100, 180 mg/L. The 24-h EC50 was determined to be > 180 mg/L.

CAS 102-71-6

The key study identified for the acute toxicity to aquatic invertebrates is a test performed by Warne et al. (1999) according to ASTM Designation E1192. Using Ceriodaphnia dubia as test species in a static procedure an EC50 of 609.88 mg/l after 48 hours of exposure was calculated related to the nominal concentrations. Hence, the chemical is considered to be acutely not harmful fo invertebrates. Other data which are available for 2,2’,2’’-nitrilotriethanol are disregarded and shout not be used for risk assessment purposes mainly due to the insufficient exposure duration.