N-(2-carboxyethyl)-N-octyl-β-alanine

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, predominantly domestic (adaptation not specified)

Duration of test (contact time):

28 d

Initial conc.:

10 mg/L

Based on:

other: carbon

Details on study design:

The test material at a concentration of 10 mg C/L was exposed to activated sewage sludge microorganisms with culture medium in sealed culture vessels in the dark at 21 °C for 28 days.

Reference substance:

benzoic acid, sodium salt

Parameter:

% degradation (CO2 evolution)

Value:

91

Sampling time:

28 d

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

The test material attained 91% degradation after 28 days.

Executive summary:

The test material at a concentration of 10 mg C/L was exposed to activated sewage sludge microorganisms with culture medium in sealed culture vessels in the dark at 21 °C for 28 days. Control solutions with inoculum and the standard material, sodium benzoate, were used for validation purposes together with a toxicity control. The test material attained 91% degradation after 28 days. By inspection of the degradation curve, the test material achieved 10 % degradation between days 4 and 5 and 60 % degradation between days 13 and 14 thereby satisfying the 10 -day window validation criterion whereby 60 % degradation must be attained within 10 days of the degradation rate exceeding 10 %. The test material can therefore be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline 301 B.

Description of key information

The test material attained 91% degradation after 28 days (OECD 301 B).

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable

Additional information

The test material at a concentration of 10 mg C/L was exposed to activated sewage sludge microorganisms with culture medium in sealed culture vessels in the dark at 21 °C for 28 days. Control solutions with inoculum and the standard material, sodium benzoate, were used for validation purposes together with a toxicity control. The test material attained 91% degradation after 28 days. By inspection of the degradation curve, the test material achieved 10 % degradation between days 4 and 5 and 60 % degradation between days 13 and 14 thereby satisfying the 10 -day window validation criterion whereby 60 % degradation must be attained within 10 days of the degradation rate exceeding 10 %. The test material can therefore be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline 301 B.