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EC number: 210-890-4 | CAS number: 625-36-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro cytogenicity / chromosome aberration study in mammalian cells
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 473 (In Vitro Mammalian Chromosome Aberration Test)
- GLP compliance:
- yes
- Type of assay:
- in vitro mammalian chromosome aberration test
Test material
- Reference substance name:
- 3-chloropropionyl chloride
- EC Number:
- 210-890-4
- EC Name:
- 3-chloropropionyl chloride
- Cas Number:
- 625-36-5
- Molecular formula:
- C3H4Cl2O
- IUPAC Name:
- 3-chloropropanoyl chloride
- Details on test material:
- - Name of test material (as cited in study report): 3-Chlorpropionsäurechlorid
- Physical state/ appearance: Yellowish to brownish liquid
- Storage conditions: 4-6 °C
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- lymphocytes: human (from heparinized human venous blood)
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254 induced rat S-9 mix
- Test concentrations with justification for top dose:
- 5, 10, 20, 40 and 60 µg/mL (without activation system), 60, 125, 250, 500, 750 µg/mL (with activation system)
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: culture medium
Controlsopen allclose all
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Remarks:
- 0.1 µg/mL
- Positive control substance:
- mitomycin C
- Remarks:
- without S-9 mix
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Remarks:
- 5 µg/mL
- Positive control substance:
- cyclophosphamide
- Remarks:
- with S-9 mix
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in medium
DURATION
- Preincubation period: 48 hours
- Exposure duration: Without metabolic activation: 24 hours; With metabolic activattion: 24 hours (After about 3 hours of incubation at 37°C, cells were washed twice with unsupplemented culture medium and then re-incubated in complete culture medium for further 21 hours).
- Fixation time (start of exposure up to fixation or harvest of cells): 72 hours
SPINDLE INHIBITOR: colcemid
STAIN: Giemsa/Titrisol solution
NUMBER OF REPLICATIONS: 2
NUMBER OF CELLS EVALUATED: 100 metaphases
DETERMINATION OF CYTOTOXICITY: mitotic index - Evaluation criteria:
- As a rule, 100 metaphases of each culture for the test substance, negative and solvent controls or 50 cells of each culture for the positive controls were analyzed for chromosome aberrations. A test substance was positive in this test if it led to a biological significant increase in the number of aberrant metaphases incl. and excl. gaps when compared to the untreated control or to the historical control.
- Statistics:
- The statistical evaluation of the data was carried out using the MUCHAN program system (BASF SE).
For each group the proportion of metaphases with aberrations was calculated.
A comparison of each dose group with the negative control group was carried out using Fisher's exact test for the hypothesis of equal proportions. This test was Bonferoni-Holm corrected over the dose groups separately for each time point and was performed one-sided.
Results and discussion
Test results
- Species / strain:
- lymphocytes: human (from heparinized human venous blood)
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- According to the results of the present study, the test substance did not cause a biological significant increase in the number of aberrant metaphases incl. and excl. gaps when compared to the untreated control or to the historical control data (2.0 - 12.0% incl. gaps or 0.5 - 4.0% excl. gaps).
No differences regarding aneuploidies (hyperploid metaphases) and polyploidies between the varous dose groups and the negative control were observed. - Remarks on result:
- other: all strains/cell types tested
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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