3-chloropropionyl chloride

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP, non guideline study, available as unpublished report, restrictions in design and/or reporting but otherwise adequate for assessment.

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1968

Report date:

1968

Reference 2

Reference Type:

other: TSCAT

Title:

TSCAT

Author:

US EPA

Year:

1992

Bibliographic source:

TSCATS: 8ECP, Doc. I.D: 88-920003052, 27.5.92; submitting organisation: BASF AG

Materials and methods

Principles of method if other than guideline:

The test substance (0.05 mL) was applied to the conjunctival sac of white Vienna rabbits. The non-treated adjacent eye served as control, to which NaCl was applied. The animals were observed several times on the treatment day and 8 days afterwards, and findings were recorded on working days. The eyes were not washed out after 24 hours after substance application. Findings were graded as described in OECD test guideline 405.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

3-chloropropionyl chloride

EC Number:

210-890-4

EC Name:

3-chloropropionyl chloride

Cas Number:

625-36-5

Molecular formula:

C3H4Cl2O

IUPAC Name:

3-chloropropanoyl chloride

Details on test material:

- Name of test material (as cited in study report): beta-Chlorpropionylchlorid

Test animals / tissue source

Species:

rabbit

Strain:

Vienna White

Details on test animals or tissues and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 2.05 and 2.13 kg

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: eye of the same animals treated with NaCl

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.05 mL

Duration of treatment / exposure:

single application

Observation period (in vivo):

8 days

Number of animals or in vitro replicates:

2

Details on study design:

SCORING SYSTEM: equivalent to the scoring system described in OECD 405 guideline.

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

cornea opacity score

Basis:

animal: #1 and #2

Time point:

other: 24 hours

Reversibility:

not fully reversible within: 8 days

Remarks on result:

other: Severe chemical burns and corneal opacity was observed throughout the study

Results 2

Irritation parameter:

chemosis score

Basis:

animal: #1 and #2

Time point:

other: 24 hour

Score:

0

Max. score:

3

Irritant / corrosive response data:

Within one hour after application the first signs of corrosion were visible in combination with slight edema. Within 24 hours corrosion was observed. The tissue underneath the eye was severely damaged. After 8 days corrosion in combination with strong supperation.