3-chloropropionyl chloride

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP non-guideline, available as unpublished report, restrictions in design and/or reporting.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1968

Report date:

1968

Materials and methods

Principles of method if other than guideline:

Five Kisslegg mice per sex per dose were exposed to 33.25, 66.5, 133, 266 and 2128 mg/kg bw of the test substance by intraperitoneal injection followed by a 14 day observation period . Besides mortality, clinical sings and bodyweights were recorded. In addition necropsy was performed on all animals.

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

3-chloropropionyl chloride

EC Number:

210-890-4

EC Name:

3-chloropropionyl chloride

Cas Number:

625-36-5

Molecular formula:

C3H4Cl2O

IUPAC Name:

3-chloropropanoyl chloride

Details on test material:

- Name of test material (as cited in study report): beta-Chlorpropionylchlorid

Test animals

Species:

mouse

Strain:

other: Kisslegg

Sex:

male/female

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

other: water and Traganth

Details on exposure:

- Test concentrations used were 0.5, 1, 2 and 20 % (G/V).
- Application volume: 5 mL/kg

Doses:

25, 50, 100, 200, 1600 cmm/kg (33.25, 66.5, 133, 266 and 2128 mg/kg bw with a relative density of 1.33)

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 42.56 mg/kg bw

Based on:

test mat.

Remarks on result:

other: Original value is 32 ccm/kg

Mortality:

- 33.25 mg/kg bw: 1/10 animals died within 48 hour. 3/10 animals after 7 days and 4/10 at the end of the observation period.
- 66.5 mg/kg bw: 8/10 animals were dead after 7 days. No additional mortality occurred.
- 133 mg/kg bw: 2/10 animals died within 48 hour. 8/10 animals after 7 days and 9/10 at the end of the observation period.
- 266 mg/kg bw: 8/10 animals were dead within 24 hours. Within 7 days all animals were dead
- 2128 mg/kg bw : Within 1 hour 3/10 were dead. After 24 hours after exposure all animals were dead.

Clinical signs:

- 2128 mg/kg bw : Immediately lying in the prone position, forced breathing, slight apathy, closed eyes.
- 266 mg/kg bw: Immediately trembling, twitching and staggering, forced breathing, long legged gait. The next days intermittent breathing, apathy and lying in the prone position.
- 133 mg/kg bw: Immediately accelerated breathing, trembling, stretching, and long legged gait. In the next days lying in the prone position, intermittent breathing, ruffled fur, sticky eyes. The one surviving animals showed no clinical signs after 9 days.
- 66.5 mg/kg bw: Immediately long legged gait, forced breathing, closed eyes. The next days ruffled fur was observed. Lying in the prone position after 7 days. After 8 days no clinical signs observed.
- 33.25 mg/kg bw: Immediately forced breathing, delayed movement of the hind limbs, eyes semi-closed. The next three days accelerated breathing and ruffled fur. After 6 days no clinical signs observed.

Body weight:

No data

Gross pathology:

Adhesions in the abdominal cavity.