Ethane- 1,2-diol, propoxylated

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2009-06-02 till 2009-06-24

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Reason / purpose for cross-reference:

reference to same study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- direct injection
- storage: Routinely, the samples were analysed immediately. Only in exceptional cases, they were stored overnight deep frozen and protected from light.

Vehicle:

no

Details on test solutions:

- direct weighing
- 105.3 mg of the test itemin 1 litre of dilution water.
- Dilution water: Reconstituted water (so-called 'M4 medium') was used for the maintenance of the test animals and the preparation of stock and test solutions of the test item.
- The pH was measured to be pH 7.7.

Test organisms (species):

Daphnia magna

Details on test organisms:

- Daphnia magna STRAUS, parthenogenetic females
- Source : Strain of Bundesgesundheitsamt Berlin
- Maintenance and Acclimatisation : A population of parthenogenetic females of synchronized age structure has been maintained for more than 15 years in the test facility under constant temperature conditions (20 +/- 1°C) at a 16 : 8 hour light-dark photoperiod (illumination: < 1000 lux). The culture water (so-called 'M4 medium') was partly renewed once a week. The Daphnia were exclusively fed with unicellular green algae (Desmodesmus subspicatus) 'ad libitum'. Mortalities of parent Daphnia during the culture period were recorded daily in a semi-quantitative way. The neonates were separated from their parent Daphnia by filtration prior to the acute test.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

16.2°dH (= 289 mg/L CaCO3)

Test temperature:

temperature range of 20.3 to 21.0 °C

pH:

8.0 - 8.1

Dissolved oxygen:

8.3 - 8.6 mg/L

Nominal and measured concentrations:

- nominal: control and 100 mg/L
- measured <3.2269 mg/L and 99.55/103.53 mg/L

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: Test concentration was analytically confirmed.

Details on results:

No toxic effects against daphnia were observed at a limit test concentration of 100 mg/L.
The results are expressed in terms of nominal concentrations. Effective concentrations correspond to 100.6% of nominal values at 0 hours, and to 101.5% of nominal values at 48 hours, respectively.

The temperature fluctuation in the course of the study exceeds 1°C which is not regarded to be relevant to the results.

Validity criteria fulfilled:

yes

Executive summary:

The acute toxicity of Ethane-1,2 -diol, propoxylated, to aquatic invertebrates (Daphnia magna) was tested according to EU method C.2 'Acute toxicity for Daphnia' (2008). No toxic effects against Daphnia magna was observed during 48 hours exposure at a limit test concentration of 100 mg/L (Currenta, 2009).

Description of key information

The acute toxicity of Ethane-1,2 -diol, propoxylated, to aquatic invertebrates (Daphnia magna) was tested according to EU method C.2 'Acute toxicity for Daphnia' (2008). No toxic effects against Daphnia magna was observed during 48 hours exposure at a limit test concentration of 100 mg/L (Currenta, 2009).

Additional information

The acute toxicity of Ethane-1,2 -diol, propoxylated, to aquatic invertebrates (Daphnia magna) was tested according to EU method C.2 'Acute toxicity for Daphnia' (2008). No toxic effects against Daphnia magna was observed during 48 hours exposure at a limit test concentration of 100 mg/L (Currenta, 2009).