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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2009-06-02 till 2009-06-24
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study
Reason / purpose for cross-reference:
reference to same study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
yes
Details on sampling:
- direct injection
- storage: Routinely, the samples were analysed immediately. Only in exceptional cases, they were stored overnight deep frozen and protected from light.
Vehicle:
no
Details on test solutions:
- direct weighing
- 105.3 mg of the test itemin 1 litre of dilution water.
- Dilution water: Reconstituted water (so-called 'M4 medium') was used for the maintenance of the test animals and the preparation of stock and test solutions of the test item.
- The pH was measured to be pH 7.7.
Test organisms (species):
Daphnia magna
Details on test organisms:
- Daphnia magna STRAUS, parthenogenetic females
- Source : Strain of Bundesgesundheitsamt Berlin
- Maintenance and Acclimatisation : A population of parthenogenetic females of synchronized age structure has been maintained for more than 15 years in the test facility under constant temperature conditions (20 +/- 1°C) at a 16 : 8 hour light-dark photoperiod (illumination: < 1000 lux). The culture water (so-called 'M4 medium') was partly renewed once a week. The Daphnia were exclusively fed with unicellular green algae (Desmodesmus subspicatus) 'ad libitum'. Mortalities of parent Daphnia during the culture period were recorded daily in a semi-quantitative way. The neonates were separated from their parent Daphnia by filtration prior to the acute test.
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Hardness:
16.2°dH (= 289 mg/L CaCO3)
Test temperature:
temperature range of 20.3 to 21.0 °C
pH:
8.0 - 8.1
Dissolved oxygen:
8.3 - 8.6 mg/L
Nominal and measured concentrations:
- nominal: control and 100 mg/L
- measured <3.2269 mg/L and 99.55/103.53 mg/L
Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Test concentration was analytically confirmed.
Details on results:
No toxic effects against daphnia were observed at a limit test concentration of 100 mg/L.
The results are expressed in terms of nominal concentrations. Effective concentrations correspond to 100.6% of nominal values at 0 hours, and to 101.5% of nominal values at 48 hours, respectively.

The temperature fluctuation in the course of the study exceeds 1°C which is not regarded to be relevant to the results.

Validity criteria fulfilled:
yes
Executive summary:

The acute toxicity of Ethane-1,2 -diol, propoxylated, to aquatic invertebrates (Daphnia magna) was tested according to EU method C.2 'Acute toxicity for Daphnia' (2008). No toxic effects against Daphnia magna was observed during 48 hours exposure at a limit test concentration of 100 mg/L (Currenta, 2009).

Description of key information

The acute toxicity of Ethane-1,2 -diol, propoxylated, to aquatic invertebrates (Daphnia magna) was tested according to EU method C.2 'Acute toxicity for Daphnia' (2008). No toxic effects against Daphnia magna was observed during 48 hours exposure at a limit test concentration of 100 mg/L (Currenta, 2009).

Key value for chemical safety assessment

Additional information

The acute toxicity of Ethane-1,2 -diol, propoxylated, to aquatic invertebrates (Daphnia magna) was tested according to EU method C.2 'Acute toxicity for Daphnia' (2008). No toxic effects against Daphnia magna was observed during 48 hours exposure at a limit test concentration of 100 mg/L (Currenta, 2009).

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