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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Endpoint:
activated sludge nitrification inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: The study was performed in accordance with sufficiently documented principles that are similar to those of currently accepted guidelines and the results are considered as reliable with restrictions.

Data source

Reference
Reference Type:
publication
Title:
Some observations on the biochemistry and inhibition of nitrification
Author:
Wood LB, Hurley BE, Matthews PJ
Year:
1981
Bibliographic source:
Water Research 15, 543-551

Materials and methods

Principles of method if other than guideline:
Solutions of the test substance were inoculated with filtered activated sludge mixed liquour and aerated for 2.5 hours at 20 to 25 °C. The inhibition of nitrification was determined by measurement of the ammonia, nitrite and nitrate concentrations.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
3-chloropropene
EC Number:
203-457-6
EC Name:
3-chloropropene
Cas Number:
107-05-1
Molecular formula:
C3H5Cl
IUPAC Name:
3-chloroprop-1-ene
Details on test material:
no data

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
no data

Test organisms

Test organisms (species):
activated sludge
Details on inoculum:
no data

Study design

Test type:
other: aeration during the test
Water media type:
freshwater
Limit test:
no
Total exposure duration:
2.5 h

Test conditions

Hardness:
no data
Test temperature:
20 to 25 °C
pH:
7.6
Dissolved oxygen:
no data, but aerated
Salinity:
no data
Nominal and measured concentrations:
no data
Details on test conditions:
no data

Results and discussion

Effect concentrations
Duration:
2.5 h
Dose descriptor:
NOEC
Effect conc.:
120 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of nitrification rate

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The NOEC for inhibition of nitrification in a mixture of activated sludge and 3-chloropropene solution was 120 mg/L in a test that was carried out over 2.5 hours.
Executive summary:

The inhibitory effect of 3-chloropropene of the nitrification in a mixture of activated sludge liquor and the test solution was tested during 2.5 hours. The mixture was aerated during the test. The NOEC based on nominal concentrations was 120 mg/L.