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EC number: 701-241-0
CAS number: -
Immobilisation Data in the
Nominal Loading Rate
Cumulative ImmobilisedDaphnia(Initial Population: 10 Per Replicate)
Table2 Cumulative Immobilisation Data in the
R2= Replicates 1 and 2
Table3 Cumulative Immobilisation Data in the
Nominal Loading Rate (mg/l)
Height of Water Column (cm)
Depth of Vortex (cm)
Observation of Vortex
*= Start of mixing
+= End of mixing
Introduction.A study was performed to assess
the acute toxicity of the test item toDaphnia magna. The
method followed that described in the OECD Guidelines for
Testing of Chemicals (April 2004) No 202, "Daphniasp,
Acute Immobilisation Test" referenced as Method C.2 of
Commission Regulation (EC) No. 440/2008.
Methods.Following a preliminary
range-finding test, twenty daphnids (2 replicates of 10 animals)
were exposed to Water Accommodated Fractions (WAFs) of the test
item over a range of nominal loading rates of 10, 18, 32, 56
and 100 mg/l for 48 hours at a temperature of 21°C to 22ºC under
static test conditions. The number of immobilisedDaphniawere
recorded after 24 and 48 hours.
The test item was known to be volatile and hence testing
was conducted in completely filled, stoppered test vessels in
order to minimise possible losses due to volatilisation.
A positive control conducted approximately every six
months used potassium dichromate as the reference item. Daphnia
magnawas exposed to an aqueous solution of the reference
item at concentrations of 0.32, 0.56, 1.0, 1.8 and 3.2 mg/l for
48 hours at a temperature of approximately 20°C under static
test conditions. Immobilisation and any adverse reactions to
exposure were recorded after 24 and 48 hours.
Results.The 48-Hour EL*50for
the test item toDaphnia magnabased on nominal loading
rates was 35 mg/l loading rate WAF with 95% confidence limits
of 30 - 41 mg/l loading rate WAF. The No Observed Effect Loading
rate was 10 mg/l loading rate WAF.
Analysis of the test preparations at 0 hours showed
measured test concentrations to range from 3.91 to 37.6 mg/l. A
decline in measured test concentrations was observed at 48 hours
in the range of 3.06 to 30.5 mg/l.
Given that these results ranged from 78 – 81% of the
0-Hour measured test concentrations it was considered that the
decline observed in the vessels which had been opened on a daily
basis was due mainly to losses through volatility.
However, given that the toxicity cannot be attributed to a
single component or a mixture of components but to the test item
as a whole, the results were based on nominal loading rates only.
The 48-Hour EC50for the reference item toDaphnia
magnabased on nominal concentrations was 0.65 mg/l with 95%
confidence limits of 0.58 – 0.72 mg/l. The No Observed Effect
Concentration was 0.32 mg/l.
Effective Loading rate
The acute toxicity of the test item to the freshwater
invertebrateDaphnia magnahas been investigated and
gave a 48-Hour EL*50value
of 35 mg/l loading rate WAF with 95% confidence limits
of 30 - 41 mg/l loading rate WAF. The No Observed Effect
Loading rate at 48 hours was 10 mg/l loading rate WAF.
Effective Loading rate
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