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Ecotoxicological information

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Short-term toxicity to fish:

In semi-static freshwater conditions, Rainbow trout (Oncorhynchus mykiss) were exposed to tertiary butyl acetate for 96h at concentrations of 56, 100, 180, 320 and 560 mg/l (measured). Sublethal effects that were seen in this test were: swimming at the surface, lying on the bottom, increased pigmentation and swimming at the surface with increased pigmentation. Under the conditions of this study the 96h LC50 is 240 mg/l and the NOEC is 56 mg/l.

Short-term toxicity to aquatic invertebrates:

In static freshwater conditions, daphnids were exposed to tertiary butyl acetate for 48h at concentrations of 100, 180, 320, 560 and 1000 mg/l (measured). Other than immobilisation, no other sublethal effects were seen. This study concludes that the 48 hour exposure of tertiary butyl acetate to Daphnia yields a 48h EC50 of 350mg/l.

Short-term toxicity to aquatic algae and cyanobacteria:

In static freshwater conditions, Pseudokirchneriella subcapitata were exposed to tertiary butyl acetate for 96h at concentrations of 10, 32, 100 and 1000 mg/l (measured). Under the conditions of this study, the 96 h ErC50 and EbC50 of tertiary butyl acetate exposure to Pseudokirchneriella subcapitata is 64 mg/l and 5.8 mg/l respectively.

Toxicity to micro-organisms:

Cell multiplication of P. putida was observed when placed in solutions with and without tertiary butyl acetate and under identical conditions. The inhibitory concentrations were determined by measuring the turbidity of bacterial suspensions in the presence and absence of tertiary butyl acetate. The toxicity threshold of tertiary butyl acetate to P.putida was determined to be 78 mg/l.