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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1995-06-06 to 1995-10-16
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to other study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
No test surrogate or analogue material
Analytical monitoring:
yes
Details on sampling:
- Concentrations: saturated test solution (SL), 62.5% SL, 39.1% SL, 24.4% SL, 15.3% SL and 9.5% SL
- Sampling method: Aliquots were taken at 0 and 48 hours
- Sample storage conditions before analysis: not reported
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Test solutions were prepared the same day the test was conducted. 100 mg of the test item per 1 litre of the test medium were stirred for 5 hours at 30°C followed by 24 hours at 20°C using a magnetic stirrer. Thereafter, the saturated solution was centrifuged for 15 minutes at 2800 g for and the solution was decanted.
- Controls: Test medium only
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): none
- Evidence of undissolved material (e.g. precipitate, surface film, etc): none reported
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Strain: STRAUS
- Source: in-house culture
- Age at study initiation (mean and range, SD): < 24 h
- selected by sieving (0.56 mm mesh)
- Method of breeding: not reported
- Feeding during test: no

ACCLIMATION
- Acclimation period: no
- Acclimation conditions (same as test or not): yes
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
No post exposure observation period
Hardness:
Test was performed in a ISO 6431 test medium.
Test temperature:
20 - 22 °C
pH:
initial: 6.46
at 48 h: 6.84 - 7.68
Dissolved oxygen:
at 48 h: 8.4 - 8.7 mg/L
Salinity:
Test was performed in a ISO 6431 test medium.
Nominal and measured concentrations:
nominal: 100% saturation (S) = 100 mg/L, 62.5% S, 39.1% S, 24.4% S, 15.3% S, 9.5% S, and 6% S (the lowest test concentration was not analytically verified since it was expected to be below the LOQ.
measured at 0 h: 106.3, 65.2, 41.8, 25.5, 17.7,10.3 mg/L
measured at 48 h: 106, 63, 36.6, 22.8, 15.1, 7 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass beaker, 250 mL, not reported
- Aeration: ISO medium was aerated to saturation prior to test
- Type of flow-through (e.g. peristaltic or proportional diluter): not applicable
- Renewal rate of test solution (frequency/flow rate): --
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- No. of vessels per vehicle control (replicates): not applicable
- Biomass loading rate: not reported

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ISO 6341
- Culture medium different from test medium: no
- Intervals of water quality measurement: 0 and 48 h

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: continuous dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobility at 24 and 48 h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 0.63
- Justification for using less concentrations than requested by guideline: not applicable
- Range finding study
- Test concentrations: 100% to 0.1% of saturation
- Results used to determine the conditions for the definitive study: yes
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
33 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% CL: not calculable
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
28 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% CL: 16 - 38 mg/L
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
10.3 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: At 48 h, 5% immobility was found at this treatment level.
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
65.2 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mobility
Results with reference substance (positive control):
- Results with reference substance valid?
- EC50/24h : 0.94 mg/L
- Test was conducted 06.09.1995
Reported statistics and error estimates:
Probit analysis

Table 1:Acute toxic effects of undecylenic acid to Daphnia magna under static exposure conditions

 

Test concentration

Immobilisation

Nominal

Measured

 

 

Saturation of test medium (%)

Test start – 0 h

(mg/L)

Test end – 48 h

(mg/L)

0 h / 48 h

(%)

24 h

(number / %)

48 h

(number / %)

Control

n.d.

n.d.

n.a.

1 / 5

2 / 10

6.0

< LOQ

< LOQ

n.a.

1 / 5

1 / 5

9.5

10.3

7

68.0

1 / 5

1 / 5

15.3

17.7

15.1

85.3

2 / 10

2 / 10

24.4

25.5

22.8

89.4

3 / 15

5 / 25

39.1

41.8

36.6

87.6

10 / 50

19 / 95

62.5

65.2

63.0

96.6

20 / 100

20 / 100

100

106.3

106.0

99.7

20 / 100

20 / 100

n.d. = not determined;

n.a. = not applicable;

LOQ = limit of quantification (7 mg/L)

Validity criteria fulfilled:
yes
Remarks:
Mortality in the control did not exceed 10%. Oxygen concentration did not fall below 2 mg/L in any treatment during the test period.
Conclusions:
A 48h-EC50 value of 28 mg/l was calculated using Probit analysis.
Executive summary:

The acute effects of undecylenic acid to the waterflea Daphnia magna were investigated in a 48 -hour test according to OECD Guideline 202, Pt. 1. Young daphnids less then 24 -hours old were exposed under static conditions to a series of concentrations initially measured as < LOQ (7 mg/L), 10.3, 17.7, 25.5, 41.8, 65.2 and 106.3 mg/L, and, aditionally, a blank control was set up. For all treatment groups, the concentration remained during the test period at >80% of initial with the exception of the second lowest for which 68% (7 mg/L) were found at 48 hours. Since this test group was not important for the EC50 calculation, the initially measured concentrations were used for calculating the toxic endpoints. At the end of the test period, immobilisation was 10%, 5%, 5%, 10%, 25%, 95%, 100% and 100% in the control, <LOQ, 10.3, 17.7, 25.5, 41.8, 65.2 and 106.3 mg/L treatment group, respectively. On basis of these findings, the 48h-EC50 value was calculated as 28 mg/L using Probit analysis.

Description of key information

 The acute effects of undecylenic acid to the water flea Daphnia magna were investigated in a 48-hour test according to OECD Guideline 202, under static conditions. The 48h-EC50 value was equal to 28 mg/L. 

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
28 mg/L

Additional information