Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 200-068-3
CAS number: 50-85-1
Table 1A.Effect of sodium salicylate
exposure on gene toxicity in CHO cells. After being exposed to the test
chemical for 3 hrs, cells was washed with sterile PBS and then incubated
for 7 days at 37°C, 5% CO2. After 7 days, cells were
re-seeded in new 6-well plates in the absence or presence of 10mM TG as
a selection agent and returned to the incubator for 14 days at 37°C, 5%
CO2. On day 15, all 6-well plates were stained with crystal
violet and the number of colonies were counted manually. The results are
presented as the total number of colonies found in the number of
independent wells analyzed (e.g. 0 colonies in 4 wells will give 0/4) (n
= 2 samples from 2 independent cultures).
diffuse colonies were found in one single well.
Table 1B.Mutation frequency in CHO
cells after 3 hrs of exposure to sodium salicylate in the absence or
presence of 4% S9 liver microsomal fraction. N/A, no colonies present in
the samples selected with TG, i.e. no mutation frequency could be
diffuse colonies were found in one single well (see Table 1A), these
diffuse colonies were not regarded as reliable and true colonies since
the cells seemed to be apoptotic.
An in vitro mammalian cell gene
mutation study was designed and conducted to determine the genotoxicity
profile of Sodium salicylate (CAS No. 54-21-7) when administered to
Chinese Hamster Ovary (CHO) cells.
In the genotoxicity test, sodium
salicylate was administered to CHO cells for 3 hrs at the dose levels of
0.0625, 0.125, 0.25 or 0.5 mM and in the absence or presence of
exogenous metabolic activation. CHO cells representing the negative
controls were exposed to the vehicle. Positive controls, such as N-ethyl-N-nitrosourea
(ENU) experiments without metabolic activation and 7,12-dimethylbenz(a)
anthracene in experiments with metabolic activation, were also included
in each test.
Only the positive control ENU gave a
clear indication of gene mutations occurring while no other treatment
gave rise to gene toxicity. Two very diffuse colonies were seen in one
well out of four at the concentration 0.0625 mM and in the presence with
4% S9 liver microsomal fraction. These diffuse colonies are not regarded
to be relevant since the two spots were only mildly colored by crystal
violet, thus indicating that it was a small cluster of apoptotic cells
taking their last breath instead of cells surviving the TG-selection.
This is further supported by the results of the higher tested
concentrations of sodium salicylate, i.e. these concentrations did not
show any evidence of diffuse or clear colonies present.
the mutation frequency was determined, a frequency of 3.08 x 10-4was
shown after a 3 hour exposure of ENU as the positive control and in the
absence of S9 liver microsomal fraction. Since no other tested
concentration of sodium salicylate in the absence or presence of S9
liver microsomal fraction resulted in colonies, we conclude that sodium
salicylate does not give rise to gene mutations when CHO cells are
exposed in vitro to the test chemical at 0, 0.0625, 0.125, 0.25
or 0.5 mM for 3 hrs.
on the results of the current study, we conclude that sodium salicylate
does not give rise to gene mutations when CHO cells are exposed to the
test chemical in vitro at 0, 0.0625, 0.125, 0.25 or 0.5 mM for 3
hrs, in the presence or abscence of metabolic activation. Therefore Sodium
salicylate was considered to be non mutagenic and hence cannot be
classified as gene mutant in vitro.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again