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Short-term Toxicity to aquatic invertebrates:

In a 48-h acute toxicity study according to OECD guideline 202, water fleas (Daphia magna) were exposedunder static conditions

to nominal test concentrations of10, 15, 20, 30, 40, 60 and 100mg/L Saponin DAB 9/10.The aim of this acute toxicity test was to assess possible effects of the test item on daphnids. Young daphnids, aged less than 24 hours at the start of the test, were exposed to the test item for a period of 48 hours. They were kept in a sample of defined water (M4 -medium) to which the test item has been added. Immobilisation was recorded at 24 and 48 hours after the test start and compared with control values. Following results were estimated: 24 -h NOEC: 30 mg/l 24 -h EC50: > 100 mg/l 24 -h EC100: > 100 mg/l 48 -h NOEC: 20 mg/l 48 -h EC50: 65 mg/l 48 -h EC100: 100 mg/l Toxicity to microorganism:

The toxicity of Saponin DAB 9/10 towards microorganism was determined according to DIN 38412 L8. Pseudomonas Putida microorganism were exposed to Saponin DAB 9/10 for 16 hours.

At the start of the test and after 16 hours the cell number was estimated by photometric determination.

Following results were estimated:

16 -h EC10: > 2500 mg/l

16 -h EC50: > 10000 mg/l

Short-term toxicity to aquatic algae:

The test was performed according to the recommendations of the OECD Guideline 201.

The purpose of this study was to determine the inhibitory effect of the test item on the growth of the fresh water unicellular green alga Scenedesmus subspicatus. Exponentially growing cultures of this algal species were exposed to various concentrations of the test item under defined conditions. The ErC50 (growth rate), ErC100 (growth rate) and NOEC was estimated for a period of 72 hours.

Following results were determined:

72 -h NOEC: 800 mg/l

72 -h EC50: > 800 mg/l

72 -h EC100: > 800 mg/l

Short-term toxicity to fish:

In a 96-h acute toxicity study according to OECD Guideline 203, golden orfs (Leuciscus Idus Melanotus) were exposed to Saponin DAB 9/10 at nominal concentrations of 15, 20, 30, 40, 60 and 80mg/l under static conditions. Following results were estimated:

96-h NOEC: 10 mg/l

96-h LC50: 38.8 mg/l

96-h LC100: 60 mg/l