Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Eye irritation

Currently viewing:

Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study but no data on GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1983
Report date:
1983

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Diethyl phosphonate
EC Number:
212-091-6
EC Name:
Diethyl phosphonate
Cas Number:
762-04-9
Molecular formula:
C4H11O3P
IUPAC Name:
diethyl phosphonate
Test material form:
other: liquid
Details on test material:
no further data

Test animals / tissue source

Species:
rabbit
Strain:
New Zealand White
Details on test animals or tissues and environmental conditions:
TEST ANIMALS
- Weight at study initiation: 3.0 kg
- Housing: individually
- Diet ad libitum
- Water ad libitum

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19-25
- Humidity (%): 40-60
- Photoperiod (hrs dark / hrs light): 12/12

Test system

Vehicle:
unchanged (no vehicle)
Controls:
other: the untreated eye served as control
Amount / concentration applied:
0.1 ml
Duration of treatment / exposure:
24 hours
Observation period (in vivo):
21 days
Number of animals or in vitro replicates:
3
Details on study design:
Each animal received 0.1 ml of the undiluted test substance into the conjunctival sac of one eye , the other eye served as control. After application the eye lids were hold together for one second . After 24 hours the treated eye was rinsed with physiological saline solution . animals were observed ffor 21 days. Findings were scored according to Draize: Cornea, conjunctiva, iris

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
cornea opacity score
Basis:
mean
Score:
2
Max. score:
4
Reversibility:
not fully reversible within: 21 days
Irritation parameter:
iris score
Basis:
mean
Score:
1
Max. score:
2
Reversibility:
fully reversible within: 7 days
Irritation parameter:
conjunctivae score
Basis:
mean
Score:
2.7
Max. score:
3
Reversibility:
not fully reversible within: 7 days
Irritation parameter:
chemosis score
Basis:
mean
Score:
2
Max. score:
4
Reversibility:
fully reversible within: 21 days
Irritant / corrosive response data:
The test substance revealed to be a strong irritant to the eyes of rabbits

Applicant's summary and conclusion

Interpretation of results:
highly irritating
Remarks:
Migrated information
Executive summary:

According to OECD TG 405 each of 3 rabbitsl received 0.1 ml of the undiluted test substance into the conjunctival sac of one eye , the other eye served as control. After application the eye lids were hold together for one second . After 24 hours the treated eye was rinsed with physiological saline solution . Animals were observed for 21 days. Findings were scored according to Draize: Cornea, conjunctiva, iris. The testsubsance proved to be a strong irritant to the eyes of rabbits. The cloudiness of the cornea in all 3 rabbits were not fully reversible within 21 days; slight conjunctival redness was observed until day 7; this effedt and all other observed effects were reversible.