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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: The report was scientifically acceptable.

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1957
Report Date:
1957
Reference Type:
publication
Title:
No information
Author:
Miller, L.C., Tainter, M.L.
Year:
1944
Bibliographic source:
Proc. Soc. Exptl. Biol. And Med., 57, 261

Materials and methods

Test guideline
Deviations:
not specified
Principles of method if other than guideline:
Method: other: see below
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent

Test animals

Species:
rat
Strain:
not specified
Sex:
male/female
Details on test animals and environmental conditions:
TEST ANIMALS- Diet: ad libitum - Water: ad libitum

Administration / exposure

Route of administration:
oral: unspecified
Vehicle:
cotton seed oil
Details on oral exposure:
Fifty young adult albino rats (5 males and 5 females/group) were fasted for 18 +/- 2 hours and given 5 graded doses (8.19 to 32.72 grams/kg) of TNPP. The sample was prepared as 50% solution in cottonseed oil and doses were administered by stomach tube.
Doses:
8.19 - 11.32 - 16.38 - 22.62 - 32.72 g/kg bw.
No. of animals per sex per dose:
50
Control animals:
other: needed for data submission
Details on study design:
- Duration of observation period following administration: 14 days- Necropsy of survivors performed: yes. Rats that died as well as survivors sacrificed at the end of the experiment were examined for evidence of gross pathology.
Statistics:
The LD50 was computed according to the method of Miller and Tainter.

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
16 200 - 21 800 mg/kg bw
Mortality:
Mortality resulted at scattered intervals over the first five days.
Clinical signs:
All rats showed evidence of abdominal pain and catharsis after dosage. Higher doses resulted in urinary incontinence and prostration.
Body weight:
Growth of survivors was normal.
Gross pathology:
Gross pathological findings included hemorrhagic lesions in the gastric mucosa and/or duodenum in a few rats that died and hemorrhagic lungs.

Applicant's summary and conclusion

Interpretation of results:
practically nontoxic
Remarks:
Migrated informationCriteria used for interpretation of results: expert judgment
Conclusions:
LD50 calculated to be 19500 +/- 3300 mg/kg. These
Executive summary:

LD50 calculated to be 19500 +/- 3300 mg/kg.