tris (4-nonylphenol, branch) phosphorous acid ester

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

other: Unpublished report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

GLP compliance:

yes

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

4-(2-methyloctyl)phenyl bis(4-nonylphenyl) phosphite

EC Number:

701-028-2

Cas Number:

26523-78-4

Molecular formula:

C45H69O3P

IUPAC Name:

4-(2-methyloctyl)phenyl bis(4-nonylphenyl) phosphite

Constituent 2

Reference substance name:

Nonylphenol

EC Number:

246-672-0

EC Name:

Nonylphenol

Cas Number:

25154-52-3

IUPAC Name:

2-nonylphenol

Test material form:

liquid

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS- Age at study initiation: 12 weeks of age - Weight at study initiation: 2.07-2.69 kg- Fasting period before study:

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

Single dermal application of 2000 mg TNPP/kg body weight for 24 hours, using a determined density of 1.1 gm/mL.TEST SITE- Area of exposure: The test substance was introduced under gauze patches, two single layers thick, and applied directly to the skin of the body surface.- % coverage: 10%- Type of wrap if used: Animals were immobilized and the patches were secured in place by wrapping the entire trunk of the animal with an impervious bandage. Test sites were secured to prevent the animals from ingesting the test substance. REMOVAL OF TEST SUBSTANCE- Washing (if done): At the completion of the exposure period, the wrapping was removed and the skin was gently wiped to remove any test substance still remaining.

Duration of exposure:

24 hours

Doses:

2000 mg/kg bw

No. of animals per sex per dose:

10

Control animals:

other: needed for data submission

Details on study design:

- Duration of observation period following administration: 14 days- Frequency of observations and weighing: Clinical examination was made at least once a day- Necropsy of survivors performed: yes, gross necropsy was performed on all animals.

Statistics:

No data

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Mortality:

None

Clinical signs:

other: No overt signs of systemic toxicity were evident during the course of the study. No erythema or edema was observed at any of the test sites.

Applicant's summary and conclusion

Interpretation of results:

practically nontoxic

Remarks:

Migrated informationCriteria used for interpretation of results: expert judgment

Conclusions:

Based on the absence of mortality, the LD50 was determined to be greater than 2000 mg/Kg.

Executive summary:

Based on the absence of mortality, the LD50 was determined to be greater than 2000 mg/Kg.