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EC number: 202-433-2 | CAS number: 95-57-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 10 - 31 Mar 1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2000
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Version / remarks:
- adopted 24 Feb 1987
- Deviations:
- no
- GLP compliance:
- yes
- Remarks:
- according to Japanese GLP standard (as described on JECDB)
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 2-chlorophenol
- EC Number:
- 202-433-2
- EC Name:
- 2-chlorophenol
- Cas Number:
- 95-57-8
- Molecular formula:
- C6H5ClO
- IUPAC Name:
- 2-chlorophenol
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: in a closed bottle at room temperature
- Stability under test conditions: stable
- Solubility and stability of the test substance in the solvent/vehicle: water solubility is 2.71%, easy soluble in acetone and DMSO
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Final dilution of a dissolved solid, stock liquid or gel: dilution in olive oil
Test animals
- Species:
- rat
- Strain:
- Crj: CD(SD)
- Remarks:
- Crj:CD(SD)IGS
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River Laboratories Japan, Inc., Yokohama, Japan
- Females (if applicable) nulliparous and non-pregnant: yes
- Age at study initiation: 5 weeks
- Weight at study initiation: 128 - 139 g (males), 108 - 120 g (females)
- Fasting period before study: 18 h prior to administration until 3 h after administration
- Housing: 5 animals of the same sex per cage
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: 6 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 23 - 26
- Humidity (%): 45 - 63
- Air changes (per hr): 12
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- olive oil
- Details on oral exposure:
- VEHICLE
- Amount of vehicle: 10 mL/kg bw
- Lot/batch no. (if required): 8123
MAXIMUM DOSE VOLUME APPLIED: 10 mL/kg bw
CLASS METHOD (if applicable)
- Rationale for the selection of the starting dose:
In a pre-test, doses of 250, 500 and 1000 mg/kg bw was administered to 3 males and females. No mortality occured during the 7 day observation period. Based on this result, the main study was performed at 500, 1000 and 2000 mg/kg bw. - Doses:
- 500, 1000 and 2000 mg/kg bw
- No. of animals per sex per dose:
- 5
- Control animals:
- yes
- Remarks:
- gavage with olive oil
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Animals were observed 5 times at the first day and daily thereafter. Individual weights were determined on Day 0 prior to administration and at Day 4, 8 and 15.
- Necropsy of survivors performed: yes
Results and discussion
- Preliminary study:
- Doses of 250, 500 and 1000 mg/kg bw were administered to 3 males and females. A control group was included. No death occurred during the following observation period (7 days). Locomotor activity was decreased in the high-dose group at the first day. According to this result, doses of 500, 1000 and 2000 mg/kg bw were applied in the main study.
Effect levelsopen allclose all
- Key result
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- > 1 000 - < 2 000 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: no male died at 1000 mg/kg bw; 3/5 males died at 2000 mg/kg bw
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: 2/5 females died at 2000 mg/kg bw
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 2 000 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: combined approach: 5/10 animals died at 2000 mg/kg bw
- Mortality:
- 500 mg/kg bw: 0/5 males and 0/5 females died
1000 mg/kg bw: 0/5 males and 0/5 females died
2000 mg/kg bw: 3/5 males and 2/5 females - Clinical signs:
- 500 and 1000 mg/kg bw: decreased locomotor activity, abnormal gait, clonic convulsion and lateral or prone position in males; decreased locomotor activity, abnormal gait and clonic convulsion in femals
2000 mg/kg bw: decreased locomotor activity and abnormal gait in males; abnormal gait in femals
all dose groups, non-surviving animals: decreased locomotor activity, clonic convulsion, lateral or prone position 15 minutes after administration - Body weight:
- 500 and 1000 mg/kg bw: body weight gains were within the normal ranges in males and females during the whole study period
2000 mg/kg bw: slightly decreased body weight gains observed in surviving animals at Day 4 - Gross pathology:
- 500 and 1000 mg/kg bw: no abnormal findings
2000 mg/kg bw:
- non-survivng animals: discoloration in spleen was found in 1/2 female
- surviving animals sacrificed at termination: no abnormal findings
Any other information on results incl. tables
Table 1. Summary of results
Dose |
Toxicological results* |
Duration of clinical signs |
Time of death |
Mortality (%) |
Males |
||||
Control |
0/0/5 |
--- |
--- |
--- |
500 |
0/4/5 |
15 min – 3 h |
--- |
0 |
1000 |
0/4/5 |
15 min – 3h |
--- |
0 |
2000 |
3/5/5 |
15 min – day 4 (surviving) |
1 – 3 h |
60 |
Females |
||||
Control |
0/0/5 |
--- |
--- |
--- |
500 |
0/3/5 |
15 min – 1 h |
--- |
0 |
1000 |
0/5/5 |
15 min – 1 h |
--- |
0 |
2000 |
2/5/5 |
15 min – day 3 (surviving) |
1 h – day 2 |
40 |
LD50 = 2000 mg/kg bw |
* first number = number of dead animals
second number = number of animals with clinical signs
third number = number of animals used
Applicant's summary and conclusion
- Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- CLP: Acute toxicity, Cat. 4, H302
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