[29H,31H-phthalocyaninato(2-)-N29,N30,N31,N32]zinc

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Orasol Blue GN

IUPAC Name:

Orasol Blue GN

Details on test material:

- Lot/batch No.: 80274742
- Analytical purity: about 98 %
- Water-solubility: insoluble

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 3000 mg test substance were suspended in 3000 mg Tween 80 by ultrasonic treatment. Subsequently the suspension was filled up with water to 30l.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Tween 80
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): 100 mg
- Evidence of undissolved material (e.g. precipitate, surface film, etc): sedimentation observed

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: rainbow trout
- Source: Forellenhof Fredelsloh, Moringen, Germany
- Length at study initiation (length definition, mean, range and SD): 4.7 - 5.7 cm
- Weight at study initiation (mean and range, SD): 1.1 - 2.1 g


ACCLIMATION
- Acclimation period: 4 weeks
- Type and amount of food: Kronen-Fisch Aminostart, Alleinfutter fur Forellenbrut, Kraftfiitterwerk Wesel GmbH, Wesel, Germany
- Feeding frequency: daily
- Health during acclimation (any mortality observed): no mortality observed

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

2.20 mmol/l

Test temperature:

14.3 - 16.0°C

pH:

8.2 - 8.9

Dissolved oxygen:

7.3 - 9.9 mg/l

Nominal and measured concentrations:

Nominal test substance concentration:
100 mg/l,
control,
solvent control

Analytically determined concentrations:
71.8 % to 83.3 % of the nominal value at test start.
21.1 % and 11.4 % of the nominal value after 24 hours.

Details on test conditions:

TEST SYSTEM
- Test vessel: aquaria
- Fill volume: 30 l
- Renewal rate of test solution (frequency/flow rate): every 24 hours
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate:

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: filtered tap water of the city of Rossdorf, Germany
- Alkalinity: 4.50 mmol/l


OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and symptoms of intoxication 2, 24, 48, 72 and 96 hours

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

>= 47 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 47 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

96 h LC50 > 100 mg/L (nominal) based on test mat.
96 h NOEC >= 100 mg/L (nominal) based on test mat.

The test substance sedimented and was not always homogeneously distributed in the test medium.