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Environmental fate & pathways

Adsorption / desorption

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Reference
Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2011-05-04 to 2011-08-11
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study
Qualifier:
according to
Guideline:
OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
Deviations:
no
GLP compliance:
yes (incl. certificate)
Remarks:
Date of inspection 2011-07-19 to 2011-07-21; Date of certificate 2011-08-31
Type of method:
HPLC estimation method
Media:
other: no data
Radiolabelling:
no
Details on study design: HPLC method:
PREPARATION OF SAMPLE
- Test material (0.1042 g) was diluted to 100 mL with acetonitrile.

PREPARATION OF DEAD TIME SOLUTION
- Dead time was determined by measuring the retention time of formamide (99.94 %; 344 mg/L in mobile phase.

DETERMINATION OF RETENTION TIME
- Sample dead time and reference standard solutions were injected in duplicate using the following HPLC parameters:
- Instrument: Aglient Technologies 1050/1100 incorporating autosampler and workstation
- Column: CN Optimal 5μ (150 x 4.6 mm id)
- Column temperature: 40 ºC
- Mobile phase: Methanol:water (55:45 v/v)
- Flow-rate: 1.0 mL/min
- Injection volume: 10 μL
- UV detector wavelength: 210 nm (dead time and reference standards); 205 nm (sample)
Type:
log Koc
Value:
3.15
Remarks on result:
other: Injection 1
Type:
log Koc
Value:
3.15
Remarks on result:
other: Injection 2
Type:
log Koc
Value:
3.15
Remarks on result:
other: Mean value
Type:
Koc
Value:
1 420
Remarks on result:
other: Adsorption coefficient
Details on results (HPLC method):
DISCUSSION
- In the absence of any dissociating functional groups, no specific manipulation of the mobile phase pH was required to ensure that the test item was analysed in a molecular form.
- Assessment of ionised forms was not applicable to the test material.

Retention times, capacity factors and Log10 Koc values for the sample.

 

Injection

Retention time (min)

Capacity factor (k)

Log10k

Log10Koc

1

4.033

1.022

9.48 x 10-3

3.15

2

4.033

1.022

9.48 x 10-3

3.15

Validity criteria fulfilled:
yes
Conclusions:
The adsorption coefficient of the test material was determined to be 1.42 x 10E3 (Log10 Koc = 3.15)

Description of key information

Log10 Koc estimated as 3.15 (OECD 121)

Key value for chemical safety assessment

Koc at 20 °C:
1 420

Additional information

Method

The determination was carried out using the HPLC screening method, designed to be compatible with Method 121 of the OECD Guidelines for Testing of Chemicals, 22 January 2001 and Method C19 Adsorption Coefficient of Commission Regulation (EC) No 440/2008 of 30 May 2008.

Conclusion

The adsorption coefficient (Koc) of the test item has been determined to be 1.42 x 103, log10Koc3.15.