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Toxicological information

Skin sensitisation

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Administrative data

Endpoint:
skin sensitisation: in vivo (LLNA)
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Screening of numerous substances; formaldehyde used as positive control; preliminary experiments in 5 independent labs

Data source

Reference
Reference Type:
publication
Title:
Interlaboratory validation of 1% pluronic l92 surfactant as a suitable, aqueous vehicle for testing pesticide formulations using the murine local lymph node assay
Author:
Boverhof DR, Wiescinski CM, Botham P, Lees D, Debruyne E, Repetto-Larsay M, Ladics G, Hoban D, Gamer A, Remmele M, Wang-Fan w, Ullmann LG, Mehta J, Billington R, Woolhiser MR
Year:
2008
Bibliographic source:
Toxicol Sci 105(1), 79-85

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)
Deviations:
no
GLP compliance:
not specified
Type of study:
mouse local lymph node assay (LLNA)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
liquid
Specific details on test material used for the study:
37% formalin (Sigma)
no further details

In vivo test system

Test animals

Species:
mouse
Strain:
CBA
Sex:
female

Study design: in vivo (LLNA)

Vehicle:
other: 1% Pluronic L92 Surfactant in water
Concentration:
0, 1, 5, 20%
No. of animals per dose:
5
Details on study design:
similar to OECD429
Positive control substance(s):
hexyl cinnamic aldehyde (CAS No 101-86-0)
Statistics:
EC3 value calculated

Results and discussion

Positive control results:
valid

In vivo (LLNA)

Resultsopen allclose all
Parameter:
EC3
Value:
3.8
Test group / Remarks:
Lab 1
Parameter:
EC3
Value:
8
Test group / Remarks:
Lab 2
Parameter:
EC3
Value:
5.6
Test group / Remarks:
Lab 3
Parameter:
EC3
Value:
8.2
Test group / Remarks:
Lab 4
Parameter:
EC3
Value:
12.3
Test group / Remarks:
Lab 5

Applicant's summary and conclusion

Interpretation of results:
other: sensitising
Conclusions:
In 5 independent laboratories positive results were obtained in the LLNA using 1% Pluronic L92 Surfactant in water as a vehicle. The EC3 values (3-fold increase in stimulation index) were 3.8-12.3%.